Klaus M. Hahn

TL;DR: A biosensor based on an engineered fibronectin monobody scaffold that can be tailored to bind different targets via high throughput screening that minimizes cell perturbation and sensitivity is enhanced by direct dye excitation.

TL;DR: Production of biologically relevant CTL escape virus variants in vivo requires selection of mutations in more than one and likely all the CTL epitopes, a low probability event.

TL;DR: This review provides a survey of non-channel proteins that have been engineered for optogenetics, using existing molecules to illustrate the advantages and disadvantages of the many imaginative new approaches that the reader can use to create light-controlled proteins.

TL;DR: A collection of prospectives is presented to provide a glimpse of the techniques that will aid in collecting, managing and utilizing information on complex cellular processes via molecular imaging tools, including: visualizing intracellular protein activity with fluorescent markers, high throughput (and automated) imaging of multilabeled cells in statistically significant numbers, and machine intelligence to analyze subcellular image localization and pattern.

TL;DR: This paper showed that Rho, Rac, and Cdc42 are not required for assembly of actin pedestals; however, they may be involved in EPEC uptake by HeLa cells.