K
Klaus M. Hahn
Researcher at University of North Carolina at Chapel Hill
Publications - 215
Citations - 16976
Klaus M. Hahn is an academic researcher from University of North Carolina at Chapel Hill. The author has contributed to research in topics: RHOA & Proto-oncogene tyrosine-protein kinase Src. The author has an hindex of 61, co-authored 210 publications receiving 15343 citations. Previous affiliations of Klaus M. Hahn include University of California, Berkeley & University of California, San Diego.
Papers
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Journal ArticleDOI
Engineered kinase activation reveals unique morphodynamic phenotypes and associated trafficking for Src family isoforms
Pei Hsuan Chu,Denis Tsygankov,Matthew E. Berginski,Onur Dagliyan,Shawn M. Gomez,Timothy C. Elston,Andrei V. Karginov,Klaus M. Hahn +7 more
TL;DR: It is demonstrated that Src family isoforms produce very different phenotypes, encompassing cell spreading, polarized motility, and production of long, thin cell extensions.
Book ChapterDOI
Spatiotemporal control of small GTPases with light using the LOV domain.
TL;DR: These findings demonstrate that cells within the border cell cluster communicate and are guided collectively, and generate versions of Rho family GTPases whose interactions with downstream effectors is controlled by light.
Book ChapterDOI
Imaging spatiotemporal dynamics of Rac activation in vivo with FLAIR.
TL;DR: The chapter describes a detailed protocol for the production of pure PBD and the process of examining Rac nucleotide state in cells using FLAIR, a system based on microinjection of a fluorescently labeled domain from p21-activated kinase into cells expressing green fluorescent protein (GFP)-Rac.
Book ChapterDOI
Digital autofocus methods for automated microscopy.
TL;DR: This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging, and hardware and software algorithms are described.
Journal ArticleDOI
Dissecting motility signaling through activation of specific Src-effector complexes
Andrei V. Karginov,Denis Tsygankov,Matthew E. Berginski,Pei Hsuan Chu,Evan D. Trudeau,Jason J. Yi,Shawn M. Gomez,Timothy C. Elston,Klaus M. Hahn +8 more
TL;DR: An approach to selectively activate a kinase in a specific protein complex or at a specific subcellular location within living cells, and within minutes is described, which reveals the effects of specific kinase pathways without time for genetic compensation.