K
Kunihiko Yoshimura
Researcher at National Institutes of Health
Publications - 11
Citations - 2777
Kunihiko Yoshimura is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Cystic fibrosis transmembrane conductance regulator & Genetic enhancement. The author has an hindex of 9, co-authored 11 publications receiving 2753 citations.
Papers
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Journal ArticleDOI
In vivo transfer of the human cystic fibrosis transmembrane conductance regulator gene to the airway epithelium
Melissa A. Rosenfeld,Kunihiko Yoshimura,Bruce C. Trapnell,Koichi Yoneyama,Eugene Rosenthal,Wilfried Dalemans,Masashi Fukayama,Joachim Bargon,L Stier,Leslie Stratford-Perricaudet,Michel Perricaudet,William B. Guggino,Andrea Pavirani,J P Lecocq,Ronald G. Crystal +14 more
TL;DR: Observations suggest the feasibility of in vivo CFTR gene transfer as therapy for the pulmonary manifestations of CF.
Journal ArticleDOI
Adenovirus-mediated transfer of a recombinant alpha 1-antitrypsin gene to the lung epithelium in vivo
Melissa A. Rosenfeld,Wolfgang Siegfried,Kunihiko Yoshimura,Koichi Yoneyama,Masashi Fukayama,L Stier,Paavo K. Paakko,Pascale Gilardi,L D Stratford-Perricaudet,Michel Perricaudet,Sophie Jallat,Andrea Pavirani,Jean-Pierre Lecocq,Ronald G. Crystal +13 more
TL;DR: The respiratory epithelium is a potential site for somatic gene therapy for the common hereditary disorders alpha 1-antitrypsin (alpha 1AT) deficiency and cystic fibrosis by infecting epithelial cells of the cotton rat respiratory tract in vitro and in vivo.
Journal ArticleDOI
Expression of the cystic fibrosis transmembrane conductance regulator gene in the respiratory tract of normal individuals and individuals with cystic fibrosis
Bruce C. Trapnell,Chin-Shyan Chu,Paavo K. Paakko,Tyrone C. Banks,Kunihiko Yoshimura,Victor J. Ferrans,Milica S. Chernick,Ronald G. Crystal +7 more
TL;DR: Estimation of CFTR gene expression quantified by limited polymerase chain reaction amplification showed that in normal individuals, CFTR mRNA transcripts are expressed in nasal, tracheal, and bronchial epithelial cells at approximately 1-2 copies per cell, more than 100-fold greater than in pharyngeal epithelium.
Journal ArticleDOI
Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer
Kunihiko Yoshimura,Melissa A. Rosenfeld,Hidenori Nakamura,Eva M. Scherer,Andrea Pavirani,Jean-Pierre Lecocq,Ronald G. Crystal +6 more
TL;DR: In vivo evaluation of beta-galactosidase activity after intratracheal administration of an E. coli lacZ gene expression plasmid vector directed by the cytomegalovirus promoter demonstrated that the airway epithelium was the major target of transfer and expression of the exogenous gene.
Patent
Method of adenoviral-medicated cell transfection
TL;DR: In this paper, an adenoviral-mediated method of transfection with nucleic acids which can be augmented through incubation of the nucleic acid with cationic agents is presented.