L
Li-Fang Chu
Researcher at Morgridge Institute for Research
Publications - 35
Citations - 2548
Li-Fang Chu is an academic researcher from Morgridge Institute for Research. The author has contributed to research in topics: Embryonic stem cell & Induced pluripotent stem cell. The author has an hindex of 17, co-authored 33 publications receiving 2011 citations. Previous affiliations of Li-Fang Chu include Center for Cell and Gene Therapy & University of Wisconsin-Madison.
Papers
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Journal ArticleDOI
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis
Zhonggang Hou,Yan Zhang,Nicholas E. Propson,Sara E. Howden,Li-Fang Chu,Erik J. Sontheimer,James A. Thomson,James A. Thomson,James A. Thomson +8 more
TL;DR: Efficient targeting of an endogenous gene in three hPSC lines using HDR is demonstrated using a distinct CRISPR-Cas system identified in Neisseria meningitidis, which is distinct from the commonly used Streptococcus pyogenes system.
Journal ArticleDOI
Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm
Li-Fang Chu,Ning Leng,Ning Leng,Jue Zhang,Zhonggang Hou,Zhonggang Hou,Daniel Mamott,David T. Vereide,Jeea Choi,Christina Kendziorski,Ron Stewart,James A. Thomson,James A. Thomson,James A. Thomson +13 more
TL;DR: It is demonstrated that KLF8 plays a pivotal role modulating mesendoderm to DE differentiation, and the strategy of combining single-cell analysis and genetic approaches can be applied to uncover novel regulators governing cell fate decisions in a variety of systems.
Journal ArticleDOI
SCnorm: robust normalization of single-cell RNA-seq data
Rhonda Bacher,Li-Fang Chu,Ning Leng,Audrey P. Gasch,James A. Thomson,Ron Stewart,Michael A. Newton,Christina Kendziorski +7 more
TL;DR: SCnorm is introduced for accurate and efficient normalization of single-cell RNA-seq data and addresses the problem of artifacts that bias downstream analyses.
Journal ArticleDOI
A statistical approach for identifying differential distributions in single-cell RNA-seq experiments.
Keegan Korthauer,Li-Fang Chu,Michael A. Newton,Yuan Li,James A. Thomson,James A. Thomson,James A. Thomson,Ron Stewart,Christina Kendziorski +8 more
TL;DR: This work presents a novel method to characterize differences in expression in the presence of distinct expression states within and among biological conditions that has higher power to detect subtle differences in gene expression distributions that are more complex than a mean shift.
Journal ArticleDOI
Ronin Is Essential for Embryogenesis and the Pluripotency of Mouse Embryonic Stem Cells
Marion Dejosez,Joshua S. Krumenacker,Laura Jo Zitur,Laura Jo Zitur,Marco Passeri,Li-Fang Chu,Zhou Songyang,James A. Thomson,Thomas P. Zwaka,Thomas P. Zwaka +9 more
TL;DR: A candidate pluripotency factor, Ronin, that possesses a THAP domain, which is associated with sequence-specific DNA binding and epigenetic silencing of gene expression is described, which suggests an epigenetic mechanism of gene repression in pluripotent cells.