L
Lishan Chen
Researcher at University of Washington
Publications - 8
Citations - 1187
Lishan Chen is an academic researcher from University of Washington. The author has contributed to research in topics: Agrobacterium tumefaciens & Agrobacterium. The author has an hindex of 6, co-authored 7 publications receiving 1152 citations.
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Journal ArticleDOI
The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58
Derek W. Wood,João C. Setubal,Rajinder Kaul,Dave E. Monks,João Paulo Kitajima,Vagner K. Okura,Yang Zhou,Lishan Chen,Gwendolyn E. Wood,Nalvo F. Almeida,Lisa Woo,Yuching Chen,Ian T. Paulsen,Jonathan A. Eisen,Peter D. Karp,Donald Bovee,Peter Chapman,James B. Clendenning,Glenda Deatherage,Will Gillet,Charles E. Grant,Tatyana Kutyavin,Ruth Levy,Meng-Jin Li,Erin K. McClelland,Anthony Palmieri,Christopher K. Raymond,Gregory Rouse,Channakhone Saenphimmachak,Zaining Wu,Pedro Romero,David E. Gordon,Shiping Zhang,Heayun Yoo,Yumin Tao,Phyllis Biddle,Mark Timothy Jung,William Krespan,Michael Perry,Bill Gordon-Kamm,Li Liao,Sun Kim,Carol A. Hendrick,Zuo-Yu Zhao,Maureen Dolan,Forrest Chumley,Scott V. Tingey,Jean-Francois Tomb,Milton P. Gordon,Maynard V. Olson,Eugene W. Nester +50 more
TL;DR: The 5.67-megabase genome of the plant pathogen Agrobacterium tumefaciens C58 consists of a circular chromosome, a linear chromosome, and two plasmids that suggest a recent evolutionary divergence.
Journal ArticleDOI
Agrobacterium VirD2 protein interacts with plant host cyclophilins
Wanyin Deng,Lishan Chen,Derek W. Wood,Tracee Metcalfe,Xiaoyou Liang,Milton P. Gordon,Luca Comai,Eugene W. Nester +7 more
TL;DR: The VirD2-cyclophilin interaction is disrupted in vitro by cyclosporin A, which also inhibits Agrobacterium-mediated transformation of Arabidopsis and tobacco, and strongly suggest that host cyclophilins play a role in T-DNA transfer.
Journal ArticleDOI
VirE1 is a specific molecular chaperone for the exported single‐stranded‐DNA‐binding protein VirE2 in Agrobacterium
Wanyin Deng,Lishan Chen,Wen-Tao Peng,Xiaoyou Liang,Susumu Sekiguchi,Milton P. Gordon,Luca Comai,Eugene W. Nester +7 more
TL;DR: VirE1 functions as a specific molecular chaperone for VirE2, the first such chaper one linked to the presumed type IV secretion system and, perhaps, maintains VirE1 in an export‐competent state, according to this report.
Journal ArticleDOI
A New Type IV Secretion System Promotes Conjugal Transfer in Agrobacterium tumefaciens
TL;DR: Data suggest that complex substrate interactions exist among the three DNA transfer systems of Agrobacterium, including the VirB system, which promotes the conjugal transfer of the IncQ plasmid RSF1010, suggesting that these products comprise a mating-pair formation system.
Journal ArticleDOI
Agrobacterium type IV secretion is a two‐step process in which export substrates associate with the virulence protein VirJ in the periplasm
TL;DR: The findings suggest that the substrate proteins localized to the periplasm may associate with the pilus in a manner that is mediated by VirJ, and suggest a two‐step process for type IV secretion in Agrobacterium.