Lucy Rutten

TL;DR: Robust single-shot vaccine protection against SARS-CoV-2 in nonhuman primates is demonstrated and vaccine-elicited neutralizing antibody titers correlated with protective efficacy, suggesting an immune correlate of protection.

TL;DR: In vitro characterization demonstrated that the introduction of stabilizing substitutions increased the ratio of neutralizing versus non-neutralizing antibody binding, suggestive for a prefusion conformation of the S protein, and the wild-type signal peptide was best suited for the correct cleavage needed for a natively folded protein.

TL;DR: In this article, the structure-based design of soluble S trimers with increased yields and stabilities, based on introduction of single point mutations and disulfide-bridges, was reported.

TL;DR: In vitro characterization demonstrated that the introduction of stabilizing substitutions increased the ratio of neutralizing versus non-neutralizing antibody binding, suggestive for a prefusion conformation of the S protein, and the wild type signal peptide was best suited for the correct cleavage needed for a natively-folded protein.

TL;DR: This work reports a universal strategy that provides for correctly folded Env trimers of high quality and yield through a repair-and-stabilize approach, and provides a means to produce prefusion-closed EnV trimers from diverse HIV-1 strains, a substantial advance for vaccine development.