M
Magdalena Eichtinger
Researcher at Harvard University
Publications - 3
Citations - 1216
Magdalena Eichtinger is an academic researcher from Harvard University. The author has contributed to research in topics: Zinc finger & Zinc finger nuclease. The author has an hindex of 3, co-authored 3 publications receiving 1171 citations.
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Journal ArticleDOI
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.
Morgan L. Maeder,Stacey Thibodeau-Beganny,Anna Osiak,David A. Wright,Reshma M. Anthony,Magdalena Eichtinger,Tao Jiang,Jonathan E. Foley,Ronnie J. Winfrey,Jeffrey A. Townsend,Erica Unger-Wallace,Jeffry D. Sander,Felix Müller-Lerch,Fengli Fu,Joseph Pearlberg,Carl Göbel,Justin P. Dassie,Shondra M. Pruett-Miller,Matthew H. Porteus,Dennis C. Sgroi,A. John Iafrate,Drena Dobbs,Paul B. McCray,Toni Cathomen,Daniel F. Voytas,J. Keith Joung +25 more
TL;DR: OP (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which is more effective than the previously published modular assembly method and provides an "open-source" method for rapidly engineering highly active zinc-finger arrays.
Journal ArticleDOI
Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly.
David A. Wright,Stacey Thibodeau-Beganny,Jeffry D. Sander,Ronnie J. Winfrey,Andrew S. Hirsh,Magdalena Eichtinger,Fengli Fu,Matthew H. Porteus,Drena Dobbs,Daniel F. Voytas,J. Keith Joung +10 more
TL;DR: A comprehensive, publicly available archive of plasmids encoding more than 140 well-characterized zinc finger modules together with complementary web-based software for identifying potential zinc finger target sites in a gene of interest is described.
Journal ArticleDOI
DNA-binding specificity is a major determinant of the activity and toxicity of zinc-finger nucleases.
Tatjana I. Cornu,Stacey Thibodeau-Beganny,Eva Guhl,Stephen Alwin,Magdalena Eichtinger,Jae Keith Joung,Toni Cathomen +6 more
TL;DR: The results of these cell-based assays reveal that the DNA-binding specificity-in addition to the affinity-is a major determinant of ZFN activity and is inversely correlated with ZFN-associated toxicity, and provide the first evidence that engineering strategies, which account for context-dependent DNA- binding effects, yield ZFs that function as highly efficient ZFNs in human cells.