D
David A. Wright
Researcher at Iowa State University
Publications - 32
Citations - 5223
David A. Wright is an academic researcher from Iowa State University. The author has contributed to research in topics: Gene & Genome editing. The author has an hindex of 21, co-authored 32 publications receiving 4837 citations. Previous affiliations of David A. Wright include University of Nebraska–Lincoln.
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Journal ArticleDOI
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.
Morgan L. Maeder,Stacey Thibodeau-Beganny,Anna Osiak,David A. Wright,Reshma M. Anthony,Magdalena Eichtinger,Tao Jiang,Jonathan E. Foley,Ronnie J. Winfrey,Jeffrey A. Townsend,Erica Unger-Wallace,Jeffry D. Sander,Felix Müller-Lerch,Fengli Fu,Joseph Pearlberg,Carl Göbel,Justin P. Dassie,Shondra M. Pruett-Miller,Matthew H. Porteus,Dennis C. Sgroi,A. John Iafrate,Drena Dobbs,Paul B. McCray,Toni Cathomen,Daniel F. Voytas,J. Keith Joung +25 more
TL;DR: OP (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which is more effective than the previously published modular assembly method and provides an "open-source" method for rapidly engineering highly active zinc-finger arrays.
Journal ArticleDOI
High-frequency modification of plant genes using engineered zinc-finger nucleases
Jeffrey A. Townsend,David A. Wright,Ronnie J. Winfrey,Fengli Fu,Morgan L. Maeder,J. Keith Joung,Daniel F. Voytas +6 more
TL;DR: High-frequency ZFN-stimulated gene targeting at endogenous plant genes, namely the tobacco acetolactate synthase genes (ALS SuRA and SuRB), for which specific mutations are known to confer resistance to imidazolinone and sulphonylurea herbicides are demonstrated.
Journal ArticleDOI
TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain
Ting Li,Sheng Huang,Wen Zhi Jiang,David A. Wright,Martin H. Spalding,Donald P. Weeks,Bing Yang +6 more
TL;DR: The creation and initial characterization of a group of rare-cutting, site-specific DNA nucleases produced by fusion of the restriction enzyme FokI endonuclease domain (FN) with the high-specificity DNA-binding domains of AvrXa7 and PthXo1 are reported.
Journal ArticleDOI
Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes
Ting Li,Sheng Huang,Xuefeng Zhao,David A. Wright,Susan Carpenter,Martin H. Spalding,Donald P. Weeks,Bing Yang +7 more
TL;DR: These studies expand the realm of verified TALEN activity from cultured human cells to an intact eukaryotic organism and suggest that low-cost, highly dependable dTALENs can assume a significant role for gene modifications of value in human and animal health, agriculture and industry.
Journal ArticleDOI
Unexpected failure rates for modular assembly of engineered zinc fingers
Cherie L. Ramirez,Jonathan E. Foley,David A. Wright,Felix Müller-Lerch,Shamim H. Rahman,Tatjana I. Cornu,Ronnie J. Winfrey,Jeffry D. Sander,Fengli Fu,Jeffrey A. Townsend,Toni Cathomen,Daniel F. Voytas,J. Keith Joung +12 more
TL;DR: In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b.