Ronnie J. Winfrey

TL;DR: OP (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which is more effective than the previously published modular assembly method and provides an "open-source" method for rapidly engineering highly active zinc-finger arrays.

TL;DR: High-frequency ZFN-stimulated gene targeting at endogenous plant genes, namely the tobacco acetolactate synthase genes (ALS SuRA and SuRB), for which specific mutations are known to confer resistance to imidazolinone and sulphonylurea herbicides are demonstrated.

TL;DR: In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b.

TL;DR: It is demonstrated that chromosome breaks created by zinc-finger nucleases greatly enhance the frequency of localized recombination in tobacco test system, suggesting that plant genome engineering through homologous recombination can now be reliably accomplished using zinc- finger nucleases.

TL;DR: A comprehensive, publicly available archive of plasmids encoding more than 140 well-characterized zinc finger modules together with complementary web-based software for identifying potential zinc finger target sites in a gene of interest is described.