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Anna Osiak
Researcher at Hannover Medical School
Publications - 3
Citations - 852
Anna Osiak is an academic researcher from Hannover Medical School. The author has contributed to research in topics: Zinc finger nuclease & Induced pluripotent stem cell. The author has an hindex of 3, co-authored 3 publications receiving 823 citations. Previous affiliations of Anna Osiak include Charité.
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Journal ArticleDOI
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.
Morgan L. Maeder,Stacey Thibodeau-Beganny,Anna Osiak,David A. Wright,Reshma M. Anthony,Magdalena Eichtinger,Tao Jiang,Jonathan E. Foley,Ronnie J. Winfrey,Jeffrey A. Townsend,Erica Unger-Wallace,Jeffry D. Sander,Felix Müller-Lerch,Fengli Fu,Joseph Pearlberg,Carl Göbel,Justin P. Dassie,Shondra M. Pruett-Miller,Matthew H. Porteus,Dennis C. Sgroi,A. John Iafrate,Drena Dobbs,Paul B. McCray,Toni Cathomen,Daniel F. Voytas,J. Keith Joung +25 more
TL;DR: OP (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which is more effective than the previously published modular assembly method and provides an "open-source" method for rapidly engineering highly active zinc-finger arrays.
Journal ArticleDOI
Targeted genome editing in pluripotent stem cells using zinc-finger nucleases
TL;DR: The use of ZFNs in murine ESCs for generating knockout alleles by NHEJ without selection or by HR employing different selection schemes is described.
Journal ArticleDOI
Selection-Independent Generation of Gene Knockout Mouse Embryonic Stem Cells Using Zinc-Finger Nucleases
Anna Osiak,Frank Radecke,Eva Guhl,Sarah Radecke,Nadine Dannemann,Fabienne Lütge,Silke Glage,Cornelia Rudolph,Tobias Cantz,Klaus Schwarz,Klaus Schwarz,Regine Heilbronn,Toni Cathomen,Toni Cathomen +13 more
TL;DR: The described ZFN-based approach represents a fast strategy for generating gene knockouts in ESCs in a selection-independent fashion that should be easily transferrable to other pluripotent stem cells.