Manfred Lindau

TL;DR: Two methods are described for estimation of passive cell parameters such as membrane capacitance, membrane conductance and access resistance in tight-seal whole cell recording by using a time domain technique and a lock-in amplifier.

TL;DR: Exocytosis of individual chromaffin granules is investigated by using cell-attached capacitance measurements, combined with electrochemical detection of catecholamines, achieved by inserting a carbon-fibre electrode into the patch pipette and finding that the fusion-pore diameter stays at <3 nm for a variable period, which can last for several seconds, before it expands.

TL;DR: In this paper, the authors investigate the fate of single secretory vesicles after fusion with the plasma membrane by measuring capacitance changes and transmitter release in rat chromaffin cells using the cell-attached patch-amperometry technique.

TL;DR: Using planar junctions between the conducting polymer PEDOT:PSS and various electrolytes, it is possible to inject common ions and directly observe their transit through the film and a straightforward estimate of the ion drift mobilities is estimated.

TL;DR: Both the proton and azide effects, which are absent in wild type, indicate that the internal donor is removed and that the reprotonation pathway is different from wild type in these mutants.