M
Marc W. T. Werten
Researcher at Wageningen University and Research Centre
Publications - 37
Citations - 1611
Marc W. T. Werten is an academic researcher from Wageningen University and Research Centre. The author has contributed to research in topics: Pichia pastoris & Self-healing hydrogels. The author has an hindex of 19, co-authored 37 publications receiving 1469 citations. Previous affiliations of Marc W. T. Werten include Radboud University Nijmegen.
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Journal ArticleDOI
High-yield secretion of recombinant gelatins by Pichia pastoris.
TL;DR: Recombinant non‐hydroxylated gelatins based on mouse type I and rat type III collagen sequences were secreted from the methylotrophic yeast Pichia pastoris, using the Saccharomyces cerevisiae α‐mating factor prepro signal to represent the highest level of heterologous protein secretion reported to date for P. pastoris.
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Secreted production of a custom-designed, highly hydrophilic gelatin in Pichia pastoris
TL;DR: Despite the fact that gelatins are highly susceptible to proteolysis because of their unfolded structure, the recombinant protein was shown to be fully intact by SDS-PAGE, N-terminal sequencing, gel filtration chromatography and mass spectrometry.
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Design and self-assembly of simple coat proteins for artificial viruses
Armando Hernandez-Garcia,Daniela J. Kraft,Anne F. J. Janssen,Paul H. H. Bomans,Nico A. J. M. Sommerdijk,Dominique M. E. Thies-Weesie,Marco E. Favretto,Roland Brock,Frits A. de Wolf,Marc W. T. Werten,Paul van der Schoot,Martien A. Cohen Stuart,Renko de Vries +12 more
TL;DR: A rational design for a self-assembling minimal viral coat protein based on simple polypeptide domains is reported, which features precise control over the cooperativity of its self-assembly with single DNA molecules to finally form rod-shaped virus-like particles.
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Covalent Microcontact Printing of Proteins for Cell Patterning
Dorota I. Rozkiewicz,Yvonne M. Kraan,Marc W. T. Werten,Frits A. de Wolf,Vinod Subramaniam,Bart Jan Ravoo,David N. Reinhoudt +6 more
TL;DR: A straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates, is described and the scope of controlled surface chemistry to direct cell adhesion is demonstrated.
Journal ArticleDOI
Precision gels from collagen-inspired triblock copolymers
Marc W. T. Werten,Helena Teles,Antoine P. H. A. Moers,Emil J.H. Wolbert,Joris Sprakel,Gerrit Eggink,Frits A. de Wolf +6 more
TL;DR: Rec recombinant gelatin-like polymers with a general design that inherently offers independent tuning of the cross-link density, melting temperature, and biocompatibility of the gel are described.