M
Markus Schirle
Researcher at Novartis
Publications - 91
Citations - 16037
Markus Schirle is an academic researcher from Novartis. The author has contributed to research in topics: Ubiquitin ligase & Protein degradation. The author has an hindex of 38, co-authored 80 publications receiving 14202 citations. Previous affiliations of Markus Schirle include University of Tübingen & University of California, Berkeley.
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Journal ArticleDOI
Proteome survey reveals modularity of the yeast cell machinery
Anne-Claude Gavin,Patrick Aloy,Paola Grandi,Roland Krause,Markus Boesche,Martina Marzioch,Christina Rau,Lars Juhl Jensen,Sonja Bastuck,Birgit Dümpelfeld,Angela Edelmann,Marie-Anne Heurtier,Verena Hoffman,Christian Hoefert,Karin Klein,Manuela Hudak,Anne-Marie Michon,Malgorzata Schelder,Markus Schirle,Marita Remor,Tatjana Rudi,Sean D. Hooper,Andreas Bauer,Tewis Bouwmeester,Georg Casari,Gerard Drewes,Gitte Neubauer,Jens Rick,Bernhard Kuster,Peer Bork,Robert B. Russell,Giulio Superti-Furga +31 more
TL;DR: This study reports the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry and provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.
Journal ArticleDOI
Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling
Shih Min A Huang,Yuji Mishina,Shanming Liu,Atwood K. Cheung,Frank Stegmeier,Gregory A. Michaud,Olga Charlat,Elizabeth Wiellette,Yue Zhang,Stephanie Wiessner,Marc Hild,Xiaoying Shi,Christine D. Wilson,Craig Mickanin,Vic E. Myer,Aleem Fazal,Ronald Tomlinson,Fabrizio C. Serluca,Wenlin Shao,Hong Cheng,Michael Shultz,Christina Rau,Markus Schirle,Judith Schlegl,Sonja Ghidelli,Stephen Fawell,Chris Lu,Daniel Curtis,Marc W. Kirschner,Christoph Lengauer,Peter Finan,John A. Tallarico,Tewis Bouwmeester,Jeffery A. Porter,Andreas Bauer,Feng Cong +35 more
TL;DR: This study uses a chemical genetic screen to identify a small molecule, XAV939, which selectively inhibits β-catenin-mediated transcription and reveals new mechanistic insights into the regulation of axin protein homeostasis, which presents new avenues for targeted Wnt pathway therapies.
Journal ArticleDOI
Quantitative mass spectrometry in proteomics: a critical review
TL;DR: This review critically examine the more commonly used quantitative mass spectrometry methods for their individual merits and discusses challenges in arriving at meaningful interpretations of quantitative proteomic data.
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A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.
Tewis Bouwmeester,Angela Bauch,Heinz Ruffner,Pierre-Olivier Angrand,Giovanna Bergamini,Karen Croughton,Cristina Cruciat,Dirk Eberhard,Julien Gagneur,Sonja Ghidelli,Carsten Hopf,Bettina Huhse,Raffaella Mangano,Anne-Marie Michon,Markus Schirle,Judith Schlegl,Markus Schwab,Martin Stein,Andreas Bauer,Georg Casari,Gerard Drewes,Anne-Claude Gavin,David B. Jackson,Gerard Joberty,Gitte Neubauer,Jens Rick,Bernhard Kuster,Giulio Superti-Furga +27 more
TL;DR: The mapping of a protein interaction network around 32 known and candidate TNF-α/NF-κB pathway components is reported by using an integrated approach comprising tandem affinity purification, liquid-chromatography tandem mass spectrometry, network analysis and directed functional perturbation studies using RNA interference.
Journal ArticleDOI
Computational prediction of proteotypic peptides for quantitative proteomics
Parag Mallick,Markus Schirle,Sharon S. Chen,Mark R. Flory,Hookeun Lee,Hookeun Lee,Daniel Martin,Jeffrey A. Ranish,Brian Raught,Robert Schmitt,Thilo Werner,Bernhard Kuster,Ruedi Aebersold +12 more
TL;DR: Using >600,000 peptide identifications generated by four proteomic platforms, it is shown that characteristic physicochemical properties of these peptides were used to develop a computational tool that can predict proteotypic peptides for any protein from any organism, for a given platform, with >85% cumulative accuracy.