Masood Z. Hadi

TL;DR: The 34 million-base-pair draft nuclear genome of the marine diatom Thalassiosira pseudonana and its 129 thousand-base pair plastid and 44 thousand base-pair mitochondrial genomes were reported in this article.

TL;DR: This work used bioinformatics to generate a list of all efflux pumps from sequenced bacterial genomes and prioritized a subset of targets for cloning, and efficiently distinguished pumps that improved survival and identified pumps that restored growth in the presence of biofuel.

TL;DR: Investigating the stability of hyperthermophilic enzymes in the presence of the IL 1-ethyl-3-methylimidazolium acetate and comparing it to the industrial benchmark Trichoderma viride (T. viride) cellulase demonstrates the potential of using IL-tolerant extremophilic cellulases for hydrolysis of IL-pretreated lignocellulosic biomass, for biofuel production.

TL;DR: This work measured the error rates for 6 DNA polymerases commonly used in PCR applications, including 3 polymerases typically used for cloning applications requiring high fidelity, and finds the lowest error rates with Pfu, Phusion, and Pwo polymerases.

TL;DR: A combination of density centrifugation and surface charge separation techniques have allowed the reproducible isolation of Golgi membranes from Arabidopsis at sufficiently high purity levels for in-depth proteomic analysis.