Matthias Machacek

TL;DR: GTPase coordination in mouse embryonic fibroblasts is examined both through simultaneous visualization of two GTPase biosensors and using a ‘computational multiplexing’ approach capable of defining the relationships between multiple protein activities visualized in separate experiments, finding that RhoA is activated at the cell edge synchronous with edge advancement, whereas Cdc42 and Rac1 are activated 2 μm behind the edge with a delay of 40 s.

TL;DR: Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks.

TL;DR: Background Interleukin (IL)‐17A has major proinflammatory activity in psoriatic lesional skin and its role in promoting wound healing is still under investigation.

TL;DR: The level set method is introduced as a numerical scheme to reconstruct continuous boundary movement in time-lapse image sequences with finite time sampling and a numerically less expensive method that satisfactorily approximates the definition of protrusion and retraction is described.

TL;DR: It is proposed that cofilin functions as a regulator of cell protrusion by modulating the spatial interaction of the lamellipodium and lamella in response to upstream signals.