Matti Kankainen

TL;DR: It is concluded that the presence of SpaC is essential for the mucus interaction of L. rhamnosus GG and likely explains its ability to persist in the human intestinal tract longer than LC705 during an intervention trial.

TL;DR: Cl cloning of the remaining pilin genes in Escherichia coli, production and purification of the recombinant proteins, and assessment of the adherence of these proteins to human intestinal mucus indicate that the SpaB and SpaF pilin subunits also exhibit substantial binding to mucus, which can be inhibited competitively in a dose-related manner.

TL;DR: Investigation of the cellular response of strain GG toward bile under defined bioreactor conditions revealed significant reduction in the abundance of a protein catalyzing the synthesis of exopolysaccharides, whereas a protein dedicated for active removal of bile compounds from the cells was up-regulated, suggesting a role for these proteins in facilitating the well founded interaction of strainGG with the host mucus in the presence of sublethal doses of biles.

TL;DR: Treating patients with solid tumors refractory to available treatments resulted in infiltration of CD8+ T cells to tumors and up-regulation of PD-L1, highlighting the potential of ONCOS-102 as an immunosensitizing agent for combinatory therapies with checkpoint inhibitors.

TL;DR: The results suggest that L. rhamnosus GG modulates its pyruvate metabolism depending on the growth pH, and proteins participating in central cellular pathways were shown to be phosphorylated, and the phosphorylation of glycolytic enzymes was found to be especially extensive.