Kirsi Savijoki

TL;DR: This review provides the current status in the research of proteolytic systems of LAB with industrial relevance and indicates that the current knowledge on stress-related proteolysis in LAB is almost exclusively based on studies on L. lactis.

TL;DR: Clp proteins are central in co‐ordinating developmental decisions and stress response in low GC Gram‐positive bacteria, thereby exerting tremendous impact on cell physiology.

TL;DR: Investigation of the cellular response of strain GG toward bile under defined bioreactor conditions revealed significant reduction in the abundance of a protein catalyzing the synthesis of exopolysaccharides, whereas a protein dedicated for active removal of bile compounds from the cells was up-regulated, suggesting a role for these proteins in facilitating the well founded interaction of strainGG with the host mucus in the presence of sublethal doses of biles.

TL;DR: The results suggest that L. rhamnosus GG modulates its pyruvate metabolism depending on the growth pH, and proteins participating in central cellular pathways were shown to be phosphorylated, and the phosphorylation of glycolytic enzymes was found to be especially extensive.

TL;DR: A secretion cassette based on the expression and secretion signals of a S-layer protein (SlpA) from Lactobacillus brevis, was constructed as mentioned in this paper, which was used as the reporter protein to determine the functionality of the Slayer signals for heterologous expression.