Institution
Valio
About: Valio is a based out in . It is known for research contribution in the topics: Lactobacillus rhamnosus & Casein. The organization has 236 authors who have published 357 publications receiving 21183 citations.
Topics: Lactobacillus rhamnosus, Casein, Probiotic, Silage, Whey protein
Papers published on a yearly basis
Papers
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TL;DR: Global and deep molecular analysis of fecal samples indicates that patients with IBS have a different composition of microbiota, and correlation analysis of the microbial groups indicated the involvement of several groups of Firmicutes and Proteobacteria in the pathogenesis of IBS.
859 citations
TL;DR: Prior to incorporating new strains into products their efficacy should be carefully assessed, and a case by case evaluation as to whether they share the safety status of traditional food-grade organisms should be made.
850 citations
TL;DR: It is concluded that the presence of SpaC is essential for the mucus interaction of L. rhamnosus GG and likely explains its ability to persist in the human intestinal tract longer than LC705 during an intervention trial.
Abstract: To unravel the biological function of the widely used probiotic bacterium Lactobacillus rhamnosus GG, we compared its 3.0-Mbp genome sequence with the similarly sized genome of L. rhamnosus LC705, an adjunct starter culture exhibiting reduced binding to mucus. Both genomes demonstrated high sequence identity and synteny. However, for both strains, genomic islands, 5 in GG and 4 in LC705, punctuated the colinearity. A significant number of strain-specific genes were predicted in these islands (80 in GG and 72 in LC705). The GG-specific islands included genes coding for bacteriophage components, sugar metabolism and transport, and exopolysaccharide biosynthesis. One island only found in L. rhamnosus GG contained genes for 3 secreted LPXTG-like pilins (spaCBA) and a pilin-dedicated sortase. Using anti-SpaC antibodies, the physical presence of cell wall-bound pili was confirmed by immunoblotting. Immunogold electron microscopy showed that the SpaC pilin is located at the pilus tip but also sporadically throughout the structure. Moreover, the adherence of strain GG to human intestinal mucus was blocked by SpaC antiserum and abolished in a mutant carrying an inactivated spaC gene. Similarly, binding to mucus was demonstrated for the purified SpaC protein. We conclude that the presence of SpaC is essential for the mucus interaction of L. rhamnosus GG and likely explains its ability to persist in the human intestinal tract longer than LC705 during an intervention trial. The presence of mucus-binding pili on the surface of a nonpathogenic Gram-positive bacterial strain reveals a previously undescribed mechanism for the interaction of selected probiotic lactobacilli with host tissues.
668 citations
TL;DR: In a randomized placebo-controlled study, 39 hypertensive patients received 150 mL/d of either L. helveticus LBK-16H fermented milk or a control product for 21 wk after a 2-week run-in period.
633 citations
TL;DR: Dietary fiber concentration and type of fat had significant effects on milk fat, and the addition of unsaturated fat resulted in marked increases in the milk fat content of trans-11-octadecenoic acid, regardless of dietary fiber concentration; however, marked milk fat depression occurred only when the low fiber diet was supplemented with uns saturated fat.
542 citations
Authors
Showing all 236 results
| Name | H-index | Papers | Citations |
|---|---|---|---|
| Jan Kok | 82 | 304 | 20002 |
| Riitta Korpela | 71 | 327 | 20160 |
| Outi Vaarala | 65 | 276 | 15473 |
| Berend Poolman | 54 | 124 | 9101 |
| Heikki Vapaatalo | 53 | 359 | 11013 |
| Reetta Satokari | 46 | 107 | 8351 |
| Girbe Buist | 45 | 98 | 6415 |
| Maija Saxelin | 34 | 47 | 8232 |
| Soile Tynkkynen | 30 | 47 | 4931 |
| Pekka Varmanen | 27 | 64 | 2744 |
| Matti Kankainen | 26 | 85 | 2815 |
| Riina A. Kekkonen | 25 | 45 | 3593 |
| Ingemar von Ossowski | 23 | 46 | 2809 |
| Justus Reunanen | 23 | 45 | 2937 |
| Anu M. Turpeinen | 23 | 42 | 1886 |