M
Meagan E Sullender
Researcher at Washington University in St. Louis
Publications - 18
Citations - 5230
Meagan E Sullender is an academic researcher from Washington University in St. Louis. The author has contributed to research in topics: CRISPR & Murine norovirus. The author has an hindex of 10, co-authored 17 publications receiving 3743 citations. Previous affiliations of Meagan E Sullender include Worcester Polytechnic Institute & Broad Institute.
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Journal ArticleDOI
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
John G. Doench,Nicolo Fusi,Meagan E Sullender,Mudra Hegde,Emma W Vaimberg,Katherine F Donovan,Ian Smith,Zuzana Tothova,Zuzana Tothova,Craig B. Wilen,Robert C. Orchard,Herbert W. Virgin,Jennifer Listgarten,David E. Root +13 more
TL;DR: Recently devised sgRNA design rules are used to create human and mouse genome-wide libraries, perform positive and negative selection screens and observe that the use of these rules produced improved results, and a metric to predict off-target sites is developed.
Journal ArticleDOI
Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
John G. Doench,Ella Hartenian,Daniel B. Graham,Zuzana Tothova,Mudra Hegde,Ian Smith,Meagan E Sullender,Benjamin L. Ebert,Ramnik J. Xavier,David E. Root +9 more
TL;DR: An online tool for the design of highly active sgRNAs for any gene of interest is provided, including a further optimization of the protospacer-adjacent motif (PAM) of Streptococcus pyogenes Cas9.
Journal ArticleDOI
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
Kendall R Sanson,Ruth E Hanna,Mudra Hegde,Katherine F Donovan,Christine Strand,Meagan E Sullender,Emma W Vaimberg,Amy Goodale,David E. Root,Federica Piccioni,John G. Doench +10 more
TL;DR: The recently-described CRISPRko library (Brunello) is more effective than previously published libraries at distinguishing essential and non-essential genes, providing approximately the same perturbation-level performance improvement over GeC KO libraries as GeCKO provided over RNAi.
Journal ArticleDOI
Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens.
Fadi J. Najm,Christine Strand,Katherine F Donovan,Mudra Hegde,Kendall R Sanson,Emma W Vaimberg,Meagan E Sullender,Ella Hartenian,Zohra Kalani,Nicolo Fusi,Jennifer Listgarten,Scott T. Younger,Bradley E. Bernstein,Bradley E. Bernstein,David E. Root,John G. Doench +15 more
TL;DR: The “Big Papi” approach described here will be widely applicable for the study of combinatorial phenotypes and generate high-complexity pooled dual-knockout libraries to identify synthetic lethal and buffering gene pairs across multiple cell types.
Journal ArticleDOI
A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair.
Joshua R. Brickner,Jennifer M. Soll,Patrick M. Lombardi,Cathrine Broberg Vågbø,Miranda C. Mudge,Clement Oyeniran,Renana Rabe,Jessica Jackson,Meagan E Sullender,Elyse M Blazosky,Andrea K. Byrum,Yu Zhao,Mark A. Corbett,Jozef Gecz,Michael Field,Alessandro Vindigni,Geir Slupphaug,Cynthia Wolberger,Nima Mosammaparast +18 more
TL;DR: A previously unrecognized ubiquitin-dependent pathway induced specifically to repair alkylation damage is revealed, shedding light on the molecular mechanism of X-linked trichothiodystrophy.