M
Michael A. Skinner
Researcher at Imperial College London
Publications - 144
Citations - 7593
Michael A. Skinner is an academic researcher from Imperial College London. The author has contributed to research in topics: Virus & Fowlpox virus. The author has an hindex of 44, co-authored 144 publications receiving 7214 citations. Previous affiliations of Michael A. Skinner include Medical Research Council & University of Reading.
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Journal ArticleDOI
Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro
Colin Dingwall,Ingemar Ernberg,Michael J. Gait,Sheila M. Green,Shaun Heaphy,Jonathan Karn,Anthony D. Lowe,Mohinder Singh,Michael A. Skinner,Robert Valerio +9 more
TL;DR: Tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes and binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays.
Journal ArticleDOI
HIV-1 tat protein stimulates transcription by binding to a U-rich bulge in the stem of the TAR RNA structure.
Colin Dingwall,Ingemar Ernberg,Michael J. Gait,Sheila M. Green,Shaun Heaphy,Jonathan Karn,Anthony D. Lowe,Mohinder Singh,Michael A. Skinner +8 more
TL;DR: There is a direct correlation between the ability of tat to bind to TAR RNA and to activate HIV transcription, and Viral LTRs carrying TAR sequences encoding any of the mutations known to produce transcripts which bind tat weakly, are not stimulated efficiently by tat in vivo.
Journal ArticleDOI
HIV-1 regulator of virion expression (Rev) protein binds to an RNA stem-loop structure located within the Rev response element region
Shaun Heaphy,Colin Dingwall,Ingemar Ernberg,Michaet J. Gait,Sheila M. Green,Jonathan Karn,Anthony D. Lowe,Mohinder Singh,Michael A. Skinner +8 more
TL;DR: A approximately 33 nucleotide fragment is protected from ribonuclease T1 digestion by the binding of Rev to RRE RNA, suggesting that Rev binds with high affinity to only a restricted region of the RRE.
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mda-5, but not RIG-I, is a common target for paramyxovirus V proteins.
Kay Childs,Nicola Stock,Craig Ross,J. Andrejeva,Louise Hilton,Michael A. Skinner,Richard E. Randall,Stephen Goodbourn +7 more
TL;DR: It is shown that 12 other paramyxoviruses also target mda-5 by a direct interaction between the conserved cysteine-rich C-terminus of their V proteins and the helicase domain of mDA-5, and that the inhibition of m da-5 function is also not restricted to mammalian cells.
Journal ArticleDOI
New model for the secondary structure of the 5' non-coding RNA of poliovirus is supported by biochemical and genetic data that also show that RNA secondary structure is important in neurovirulence.
Michael A. Skinner,Vincent R. Racaniello,Glynnis Dunn,Julian Cooper,P.D. Minor,Jeffery W. Almond +5 more
TL;DR: The sequence of a neurovirulent revertant of an attenuated mutant provides additional evidence for an interaction between a region known to be important for neurovirulence, sequence 471-483, and nucleotides 528 to 538.