M
Michael L. Nielsen
Researcher at University of Copenhagen
Publications - 134
Citations - 15189
Michael L. Nielsen is an academic researcher from University of Copenhagen. The author has contributed to research in topics: Proteome & Mass spectrometry. The author has an hindex of 48, co-authored 123 publications receiving 13104 citations. Previous affiliations of Michael L. Nielsen include University of Copenhagen Faculty of Health Sciences & University of Southern Denmark.
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Journal ArticleDOI
Mapping Physiological ADP-Ribosylation Using Activated Ion Electron Transfer Dissociation.
Sara C. Buch-Larsen,Ivo A. Hendriks,Jean M. Lodge,Martin Rykær,Benjamin Furtwängler,Evgenia Shishkova,Michael S. Westphall,Joshua J. Coon,Michael L. Nielsen +8 more
TL;DR: Under physiological conditions, activated ion electron transfer dissociation (AI-ETD) identifies 450 ADPr sites on low-abundant proteins, including in vivo cysteine modifications on poly(ADP-ribosyl)polymerase (PARP) 8 and tyrosine modificationson PARP14, hinting at specialist enzymatic functions for these enzymes.
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SUMOylation promotes protective responses to DNA-protein crosslinks
Nikoline Borgermann,Leena Ackermann,Petra Schwertman,Ivo A. Hendriks,Karen L. Thijssen,Julio C Y Liu,Hannes Lans,Michael L. Nielsen,Niels Mailand +8 more
TL;DR: It is demonstrated that ACRC/GCNA family SprT proteases interact with SUMO and establish important physiological roles of Caenorhabditis elegans GCNA‐1 and SUMOylation in promoting germ cell and embryonic survival upon DPC formation and provides first global insights into signaling responses to DPCs.
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CDK-mediated activation of the SCFFBXO28 ubiquitin ligase promotes MYC-driven transcription and tumourigenesis and predicts poor survival in breast cancer
Diana Cepeda,Hwee-Fang Ng,Hamid Sharifi,Salah Mahmoudi,Vanessa Soto Cerrato,Erik Fredlund,Kristina Magnusson,Helen Nilsson,Alena Malyukova,Juha Rantala,Daniel Klevebring,Francesc Viñals,Nimesh Bhaskaran,Siti Mariam Zakaria,Aldwin Suryo Rahmanto,Stefan Grotegut,Michael L. Nielsen,Cristina Al-Khalili Szigyarto,Dahui Sun,Mikael Lerner,Sanjay Navani,Martin Widschwendter,Mathias Uhlén,Karin Jirström,Fredrik Pontén,James A. Wohlschlegel,Dan Grandér,Charles Spruck,Lars-Gunnar Larsson,Olle Sangfelt +29 more
TL;DR: It is suggested that SCFFBxO28 plays an important role in transmitting CDK activity to MYC function during the cell cycle, emphasizing the CDK‐FBXO28‐MYC axis as a potential molecular drug target in MYC‐driven cancers, including breast cancer.
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Experiences and perspectives of MALDI MS and MS/MS in proteomic research
TL;DR: It is found that MALDI peptide mass fingerprinting alone is not sufficiently selective for current demands in proteomic research, however, the combination of MAL DI with tandem mass spectrometry such as joining a quadrupole with a TOF analyser pairs highly parallel analysis with high selectivity.
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Advances in characterizing ubiquitylation sites by mass spectrometry.
TL;DR: This review focuses on the technical advances in the mass spectrometry-based characterization of ubiquitylation sites, which have recently involved the large-scale identification of ubiquitin sites by peptide-level enrichment strategies.