M
Michael L. Nielsen
Researcher at University of Copenhagen
Publications - 134
Citations - 15189
Michael L. Nielsen is an academic researcher from University of Copenhagen. The author has contributed to research in topics: Proteome & Mass spectrometry. The author has an hindex of 48, co-authored 123 publications receiving 13104 citations. Previous affiliations of Michael L. Nielsen include University of Copenhagen Faculty of Health Sciences & University of Southern Denmark.
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Journal ArticleDOI
Electronic excitation gives informative fragmentation of polypeptide cations and anions
Kim F. Haselmann,Bogdan A. Budnik,Frank Kjeldsen,Michael L. Nielsen,Jesper V. Olsen,Roman A. Zubarev +5 more
TL;DR: In this paper, a Fourier transform mass spectrometer was used to compare the performance of hot-electron capture dissociation (HECD) and electron-detachment dissociation(EDD) with vibrational excitation (VE) techniques such as collisional activation.
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Chemical genetics and proteome-wide site mapping reveal cysteine MARylation by PARP-7 on immune-relevant protein targets.
Kelsie M Rodriguez,Sara C. Buch-Larsen,Ilsa T Kirby,Ivan Rodriguez Siordia,David Hutin,Marit Rasmussen,Denis M. Grant,Larry L. David,Jason Matthews,Jason Matthews,Michael L. Nielsen,Michael S. Cohen +11 more
TL;DR: In this paper, the authors combine chemical genetics, proximity labeling, and proteome-wide amino acid ADP-ribosylation site profiling for identifying the direct targets and sites of PARP-7-mediated MARylation in a cellular context.
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Peptide end sequencing by orthogonal MALDI tandem mass spectrometry.
TL;DR: A high-sensitivity/high-throughput system based on orthogonal MALDI tandem mass spectrometry (o-MALDI) and the automated recognition of fragments corresponding to the N- and C-terminal amino acid residues that is sufficient to uniquely identify a protein from gel samples in the low silver-stained range is described.
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Tandem MALDI/EI ionization for tandem Fourier transform ion cyclotron resonance mass spectrometry of polypeptides
TL;DR: The electronic excitation dissociation (EED) as mentioned in this paper was proposed to capture low energy electrons in the tandem ionization (TI) technique, in which protonated polypeptides [M+H]+ produced by matrix-assisted laser desorption ionization were further ionized inside a Fourier transform (FT) mass spectrometer by >10eV electrons.
Journal ArticleDOI
Serine-linked PARP1 auto-modification controls PARP inhibitor response.
Evgeniia Prokhorova,Florian Zobel,Rebecca Smith,Siham Zentout,Ian Gibbs-Seymour,Kira Schützenhofer,Alessandra Peters,Joséphine Groslambert,Valentina Zorzini,Thomas Agnew,John Brognard,Michael L. Nielsen,Dragana Ahel,Sébastien Huet,Sébastien Huet,Marcin J. Suskiewicz,Ivan Ahel +16 more
TL;DR: In this article, the authors identify three serine residues within PARP1 (499, 507, and 519) as key sites whose efficient HPF1-dependent modification counters PARP 1 trapping and contributes to inhibitor tolerance.