Cristina Al-Khalili Szigyarto

TL;DR: In this paper, a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level.

TL;DR: A quantitative transcriptomics analysis (RNA-Seq) is used to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody-based profiling of the same tissues.

TL;DR: A new publicly available database containing, in the first version, ∼400,000 high resolution images corresponding to more than 700 antibodies toward human proteins, providing a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues.

TL;DR: A new version (4.0) of the Human Protein Atlas has been developed in a genecentric manner with the inclusion of all human genes and splice variants predicted from genome efforts together with a visualization of each protein with characteristics such as predicted membrane regions, signal peptide, and protein domains and new plots showing the uniqueness of every fraction of eachprotein toward all other human proteins.

TL;DR: It is shown that antibodies specific to human proteins can be generated in a high‐throughput manner involving stringent affinity purification using recombinant protein epitope signature tags (PrESTs) as immunogens and affinity‐ligands.