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Michael R. Sussman

Researcher at University of Wisconsin-Madison

Publications -  195
Citations -  21193

Michael R. Sussman is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Arabidopsis & Arabidopsis thaliana. The author has an hindex of 73, co-authored 191 publications receiving 19330 citations. Previous affiliations of Michael R. Sussman include Wisconsin Alumni Research Foundation & Yale University.

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Expression of a translationally fused TAP-tagged plasma membrane proton pump in Arabidopsis thaliana.

TL;DR: Transgenic Arabidopsis lines are generated in which a TAP-tagged AHA1 transgene has complemented all essential endogenous AHA 1 and AHA2 functions and it is shown that these plants can be used to purify A HA1 protein and to identify in planta interacting proteins by mass spectrometry.
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In vivo cross-linking supports a head-to-tail mechanism for regulation of the plant plasma membrane P-type H+-ATPase.

TL;DR: In vivo cross-linking experiments with a photoreactive unnatural amino acid, p-benzoylphenylalanine, and tandem MS are used to obtain direct evidence that the C-terminal regulatory domain interacts with amino acids located within the N-terminAL actuator domain, consistent with a “head-to-tail” organization of ATPase monomers.
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Probing a Plant Plasma Membrane Receptor Kinase’s Three-Dimensional Structure Using Mass Spectrometry-Based Protein Footprinting

TL;DR: The results of mass spectrometry-based footprinting methods provide a framework for understanding ligand-induced changes in solvent accessibility and their positions within the three-dimensional structure of a plant receptor kinase.

The Phaeodactylum genome reveals the dynamic nature and multi-lineage evolutionary history of diatom genomes

TL;DR: The complete genome sequence of the pennate diatom Phaeodactylum tricornutum is reported and compared with that of T. pseudonana to clarify evolutionary origins, functional significance and ubiquity of these features throughout diatoms.