N
Nadja Karl
Researcher at University of Giessen
Publications - 17
Citations - 902
Nadja Karl is an academic researcher from University of Giessen. The author has contributed to research in topics: Coronavirus & RNA. The author has an hindex of 11, co-authored 16 publications receiving 682 citations. Previous affiliations of Nadja Karl include Queen's University Belfast & University of Würzburg.
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Journal ArticleDOI
Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication
Eveline Kindler,Eveline Kindler,Cristina Gil-Cruz,Julia Spanier,Yize Li,Jochen Wilhelm,Huib H. Rabouw,Roland Züst,Mihyun Hwang,Philip V'kovski,Philip V'kovski,Hanspeter Stalder,Hanspeter Stalder,Sabrina Marti,Sabrina Marti,Matthias Habjan,Luisa Cervantes-Barragan,Ruth Elliot,Nadja Karl,Christina Gaughan,Frank J. M. van Kuppeveld,Robert H. Silverman,Markus Keller,Burkhard Ludewig,Cornelia C. Bergmann,John Ziebuhr,Susan R. Weiss,Ulrich Kalinke,Volker Thiel,Volker Thiel +29 more
TL;DR: It is shown that the coronavirus endonuclease (EndoU) activity is key to prevent early induction of double-stranded RNA (dsRNA) host cell responses, which suggests an evolutionary conserved viral function that has evolved to prevent RNA-based sensing of infection in vertebrate hosts.
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Selective Replication of Coronavirus Genomes That Express Nucleocapsid Protein
TL;DR: It is found that human coronavirus 229E (HCoV-229E) vector RNAs that lack the N gene were greatly impaired in their ability to replicate, whereas the transcription of subgenomic mRNA from these vectors was easily detectable.
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Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vivo.
Scott E. Coley,Ehud Lavi,Stanley G. Sawicki,Li Fu,Barbara Schelle,Nadja Karl,Stuart G. Siddell,Volker Thiel +7 more
TL;DR: A reverse genetic system for MHV based upon the cloning of a full-length genomic cDNA in vaccinia virus is described, showing that the recombinant virus generated from cloned cDNA replicates to the same titers as the parental virus in cell culture.
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The NF-κB-dependent and -independent transcriptome and chromatin landscapes of human coronavirus 229E-infected cells
Michael Poppe,Sascha Wittig,Liane Jurida,Marek Bartkuhn,Jochen Wilhelm,Helmut Müller,Knut Beuerlein,Nadja Karl,Sabin Bhuju,John Ziebuhr,M. Lienhard Schmitz,Michael Kracht +11 more
TL;DR: The study shows that cytoplasmic RNA viruses fine-tune NF-κB signaling at multiple levels and profoundly reprogram the host cellular chromatin landscape, thereby orchestrating the timely coordinated expression of genes involved in multiple signaling, immunoregulatory and metabolic processes.
Journal ArticleDOI
Coronavirus replication-transcription complex: Vital and selective NMPylation of a conserved site in nsp9 by the NiRAN-RdRp subunit.
Heiko Slanina,Ramakanth Madhugiri,Ganesh Bylapudi,Karin Schultheiß,Nadja Karl,Anastasia A. Gulyaeva,Alexander E. Gorbalenya,Alexander E. Gorbalenya,Uwe Linne,John Ziebuhr +9 more
TL;DR: In this article, it was shown that the NiRAN-RdRp has Mn2+-dependent NMPylation activity that catalyzes the transfer of a single NMP to the cognate nsp9 by forming a phosphoramidate bond with the primary amine at the nsp 9 N terminus (N3825).