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Nediljko Budisa
Researcher at University of Manitoba
Publications - 244
Citations - 7194
Nediljko Budisa is an academic researcher from University of Manitoba. The author has contributed to research in topics: Amino acid & Genetic code. The author has an hindex of 43, co-authored 230 publications receiving 6376 citations. Previous affiliations of Nediljko Budisa include Yale University & Max Planck Society.
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High‐level Biosynthetic Substitution of Methionine in Proteins by its Analogs 2‐Aminohexanoic Acid, Selenomethionine, Telluromethionine and Ethionine in Escherichia coli
Nediljko Budisa,Boris Steipe,Boris Steipe,Pascal Demange,Christoph Eckerskorn,Josef Kellermann,Robert Huber +6 more
TL;DR: This work describes the first biosynthetic high-level substitution of methionine by 2-aminohexanoic acid (norleucine), ethionine and telluromethionine in a protein, and confirms the replacement has been confirmed by electrospray mass spectroscopy, amino acid analysis and X-ray structural analysis.
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Prolegomena to Future Experimental Efforts on Genetic Code Engineering by Expanding Its Amino Acid Repertoire
TL;DR: Important experiments provided solid evidence that the natural protein translation machinery can be reprogrammed to encode genetically a vast number of non-coded (i.e. noncanonical) amino acids.
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Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability
Nicole Nischan,Henry D. Herce,Henry D. Herce,Francesco Di Natale,Nina Bohlke,Nediljko Budisa,M. Cristina Cardoso,Christian P. R. Hackenberger,Christian P. R. Hackenberger +8 more
TL;DR: The findings reveal that the cyclic-CPP-GFP conjugates are internalized into live cells with immediate bioavailability in the cytosol and the nucleus, whereas linear CPP analogues do not confer GFP transduction.
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Expansion of the genetic code enables design of a novel "gold'' class of green fluorescent proteins
Jae Hyun Bae,Marina Rubini,Gregor Jung,Georg Wiegand,Markus H. J. Seifert,M. Kamran Azim,Jeong-Sun Kim,Andreas Zumbusch,Tad A. Holak,Luis Moroder,Robert Huber,Nediljko Budisa +11 more
TL;DR: An expansion of efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant, which possesses a red shift in emission unprecedented for any avFP.
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Fluoroprolines as Tools for Protein Design and Engineering
TL;DR: The preference of the peptidyl-fluoroproline amide bond for the cis or trans conformation in the model compounds N-acetyl-4-fluorschmidt methyl esters fully correlates with the thermostability of the related mutants of the model protein barstar.