Neville E. Sanjana

Abstract: Embodiments disclosed herein provide methods, including computer-implemented methods, for designing guide sequence which may be incorporated into custom, large scale guide sequence libraries. The methods require only a list of target genes as input and utilize on target and off target scores to generate an optimal set of guide sequences for a set of target genes. In certain embodiments, the methods may also utilize multi-tissue RNA-sequencing data and/or protein annotation to design targets to genes that are highly expressed and/or contain a functional protein domain. The invention further comprises guide libraries, cells comprising said guide libraries. Computer-implemented embodiments further improve computer system function by reducing excessive user wait time through the use of data structures that reduce search from linear to logarithmic time.

TL;DR: For example, the authors describes a scenario in which a person is confronted with a situation in which he or she is unable to find a suitable solution to the problem of having insufficient resources.

TL;DR: The results suggest that ZBTB11 and ZFP131 maintain pluripotency by preventing premature expression of pro-differentiation genes and present a generalizable framework to maintain cellular potency.

TL;DR: In this paper, a patent for "sistemas de componente crispr-cas, ma'todos e composia'a•es for manipulaa•a•o de sequasncia" is presented.