N
Nicholas R.F. Hannan
Researcher at University of Nottingham
Publications - 42
Citations - 4409
Nicholas R.F. Hannan is an academic researcher from University of Nottingham. The author has contributed to research in topics: Induced pluripotent stem cell & Stem cell. The author has an hindex of 21, co-authored 37 publications receiving 3865 citations. Previous affiliations of Nicholas R.F. Hannan include Wellcome Trust & University of Cambridge.
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Journal ArticleDOI
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
Kosuke Yusa,S. Tamir Rashid,Helene Strick-Marchand,Helene Strick-Marchand,Ignacio Varela,Pei-Qi Liu,David Paschon,Elena Miranda,Elena Miranda,Adriana Ordóñez,Nicholas R.F. Hannan,Foad J. Rouhani,Foad J. Rouhani,Sylvie Darche,Sylvie Darche,Graeme J.M. Alexander,Stefan J. Marciniak,Noemi Fusaki,Mamoru Hasegawa,Michael C. Holmes,James P. Di Santo,James P. Di Santo,David A. Lomas,Allan Bradley,Ludovic Vallier +24 more
TL;DR: This work shows that a combination of zinc finger nucleases (ZFNs) and piggyBac technology in human iPSCs can achieve biallelic correction of a point mutation in the α1-antitrypsin (A1AT, also known as SERPINA1) gene that is responsible for α1
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Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells
S. Tamir Rashid,Sébastien Corbineau,Nicholas R.F. Hannan,Stefan J. Marciniak,Elena Miranda,Graeme J.M. Alexander,Isabel Huang-Doran,Julian L. Griffin,Lars Ährlund-Richter,Jeremy N. Skepper,Robert K. Semple,Anne Weber,David A. Lomas,Ludovic Vallier +13 more
TL;DR: These patient-derived hepatocytes demonstrate that it is possible to model diseases whose phenotypes are caused by pathological dysregulation of key processes within adult cells, and a simple and effective platform for hepatocyte generation from patient-specific human iPS cells.
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Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development
Thomas Touboul,Nicholas R.F. Hannan,Sébastien Corbineau,Amelie Martinez,Clémence Martinet,Sophie Branchereau,Sylvie Mainot,Helene Strick-Marchand,Roger A. Pedersen,James P. Di Santo,Anne Weber,Ludovic Vallier +11 more
TL;DR: A robust and efficient method to differentiate pluripotent stem cells into hepatic cells, which exhibit characteristics of human hepatocytes are reported, which should facilitate the development of clinical grade hepatocytes for transplantation and for research on drug discovery.
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Transplantation of Expanded Fetal Intestinal Progenitors Contributes to Colon Regeneration after Injury
Robert P. Fordham,Shiro Yui,Shiro Yui,Nicholas R.F. Hannan,Christoffer Soendergaard,Alison Madgwick,Pawel J. Schweiger,Ole Haagen Nielsen,Ludovic Vallier,Roger A. Pedersen,Tetsuya Nakamura,Mamoru Watanabe,Kim B. Jensen,Kim B. Jensen +13 more
TL;DR: It is shown that human and mouse fetal intestine contains proliferative, immature progenitors, which can be expanded in vitro as Fetal Enterospheres (FEnS), which contribute to regeneration of colonic epithelium by forming epithelial crypt-like structures expressing region-specific differentiation markers.
Journal ArticleDOI
Production of hepatocyte-like cells from human pluripotent stem cells
Nicholas R.F. Hannan,Charis-Patricia Segeritz,Thomas Touboul,Ludovic Vallier,Ludovic Vallier +4 more
TL;DR: A 25-d protocol to direct the differentiation of human pluripotent stem cells into a near-homogenous population of hepatocyte-like cells and expresses genes in a chronological manner similar to that described during in vivo hepatic development is described.