Niels A W de Kok

TL;DR: Local differences in the chemical composition of PG are reported in the Gram‐positive rod‐shaped model organism Bacillus subtilis indicating either a lower local degree of PG crosslinking or a difference in PG composition, which could be a topological marker for other proteins.

TL;DR: This study shows that although B. subtilis ATCC 6633 and 168 are able to produce various processed lantibiotic peptides, an evident advantage of using either the 8-fold protease-deficient strain WB800 or the genome-minimized B. subilis 168 strain PG10 is the lack of extracellular serine protease activity, which means potential toxicity toward the production host is prevented.

TL;DR: Biochemical characterization revealed that both GtfX and GtfY showed GtfB-like 4,6-α-GT activity, cleaving ( α1→4) linkages and catalyzing the synthesis of (α1→6) linkaged products, but they differ in product specificity.

TL;DR: In this article, a nuclease-defective mutant of Cas9 (dCas9) was fused to a highly active tripartite activator VP64-p65-Rta (VPR) to allow for sgRNA directed targeted gene regulation.

TL;DR: These structural and functional studies provide not only an understanding of lipid biosynthesis by substrate-specific lipid-modifying enzymes but also insights into the mechanisms of lipid membrane remodeling and adaptation.