Showing papers by "Osamu Matoba published in 2021"

Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target.

Abstract: Sustained neuropathic pain from injury or inflammation remains a major burden for society. Rodent pain models have informed some cellular mechanisms increasing neuronal excitability within the spinal cord and primary somatosensory cortex (S1), but how activity patterns within these circuits change during pain remains unclear. We have applied multiphoton in vivo imaging and holographic stimulation to examine single S1 neuron activity patterns and connectivity during sustained pain. Following pain induction, there is an increase in synchronized neuronal activity and connectivity within S1, indicating the formation of pain circuits. Artificially increasing neuronal activity and synchrony using DREADDs reduced pain thresholds. The expression of N-type voltage-dependent Ca2+ channel subunits in S1 was increased after pain induction, and locally blocking these channels reduced both the synchrony and allodynia associated with inflammatory pain. Targeting these S1 pain circuits, via inhibiting N-type Ca2+ channels or other approaches, may provide ways to reduce inflammatory pain.

Single-shot common-path off-axis digital holography: applications in bioimaging and optical metrology [Invited].

Abstract: The demand for single-shot and common-path holographic systems has become increasingly important in recent years, as such systems offer various advantages compared to their counterparts. Single-shot holographic systems, for example, reduce computational complexity as only a single hologram with the object information required to process, making them more suitable for the investigation of dynamic events; and common-path holographic systems are less vibration-sensitive, compact, inexpensive, and high in temporal phase stability. We have developed a single-shot common-path off-axis digital holographic setup based on a beam splitter and pinhole. In this paper, we present a concise review of the proposed digital holographic system for several applications, including the quantitative phase imaging to investigate the morphological and quantitative parameters, as a metrological tool for testing of micro-optics, industrial inspection and measurement, and sound field imaging and visualization.

Multimodal Microscopy: Fast Acquisition of Quantitative Phase and Fluorescence Imaging in 3D Space

Abstract: Multimodal optical microscopy combines different optical and physiological principles working together to obtain rich perspectives from a single biological event. In this paper, the recent advances in multimodal microscopy systems based on off-axis digital holography and transport-of-intensity equation are reviewed. Both methods used the common-path configuration, and realized simultaneous quantitative phase and fluorescence imaging in 3D space. Feasibility checks with fluorescent micro-beads and live plant cells are presented. The results show that both methods are capable of fast acquisition of the quantitative phase and fluorescence information with digital refocusing ability.

High-speed imaging of the sound field by parallel phase-shifting digital holography

Abstract: Sound field imaging techniques have been found very useful for acoustic designs. Building on this idea, innovative techniques are needed and presented in this paper, where we report on developed imaging of the sound field radiated from speakers by parallel phase-shifting digital holography. We adopted an ultrasonic wave radiated from a speaker for an object. The phase distribution of the light wave was modulated by the sound field radiated from the speaker. The modulated phase distribution was recorded in the form of multiplexed phase-shifted holograms at the frame rate of 100,000 fps. A 40,000 Hz sound field radiated from a speaker is used as an observation target. Our proposed method can implement the imaging of the sound field successfully. Also, in order to demonstrate the digital refocusing capability of digital holography, we set two speakers, whose difference in depth positions was 6.6 cm, as a long-depth object. We demonstrated the digital refocusing on the two speakers along with the capability of measuring the positions of the objects. Furthermore, we succeeded in imaging of 40,000 Hz and 41,000 Hz sound fields radiated from the two speakers. The presented experimental results showed that parallel phase-shifting digital holography is very useful and suitable for sound field imaging.

Multimodal sound field imaging using digital holography [Invited].

Abstract: Sound is an important invisible physical phenomenon that needs to be explained in several physical and biological processes, along with visual phenomena. For this purpose, multiparameter digital holography (DH) has been proposed to visualize both features simultaneously due to the phase and amplitude reconstruction properties of DH. In this paper, we present a brief review on sound field imaging techniques with special focus on the multiparameter imaging capability of DH for visualizing sound and visual features. The basic theory and several experimental results with very high-speed recordings are also presented to demonstrate sound field imaging for the audible range as well as in the ultrasound range.

Multi-Physical Parameter Cross-Sectional Imaging of Quantitative Phase and Fluorescence by Integrated Multimodal Microscopy

Abstract: Integrated multimodal cross-sectional or volumetric imaging techniques give us fruitful information to understand the behavior or status of target objects such as biological samples. Most of the reported systems for this purpose are either time consuming due to scanning or use additional reference beams such as in interferometry. Therefore, fast, simple, highly efficient, and powerful multimodal imaging systems that can perform cross-sectional imaging with simple algorithms are worth to be investigated. In this paper, a multimodal technique for cross-sectional quantitative phase and fluorescence imaging with computational microscopy is presented. We combine cross-sectional fluorescence and quantitative phase imaging by using the transport of intensity equation (TIE) and numerical wave propagation. The amplitude and phase of the fluorescence light wave with partially spatial coherence are obtained from three defocused intensity patterns. The proposed hybrid imaging system is simple, compact, and non-iterative. We present experimental results of microbeads and fluorescent protein-labeled living cells of the moss Physcomitrella patens to demonstrate the performance of the proposed imaging system.

2D full-field displacement and vibration measurements of specularly reflecting surfaces by two-beam common-path digital holography.

Abstract: A new, to the best of our knowledge, configuration of common-path off-axis digital holography is proposed for simultaneous evaluation of out-of-plane and in-plane displacements of the vibrating object. The object is illuminated from two different directions, and each illumination interferes with its corresponding reference beam generated near the object, resulting in two independent holograms that are spatially multiplexed in a single camera image. Two multiplexed holograms, at undeformed and deformed states of the object, are recorded and processed to obtain the out-of-plane and in-plane displacements simultaneously. The proposed digital holographic system has the advantage of a simple and compact optical setup, is less sensitive to environmental disturbances, and has high temporal phase stability. The two-dimensional (z,x) full-field amplitude and phase vibration analysis of a perfect specularly reflecting surface are also demonstrated by the proposed holographic system. The experimental results authenticate the feasibility of the proposed system and reveal its unique advantages. The proposed digital holographic system, owing to simple and compact geometry and providing several advantages over other two-channel holographic systems, may find a wide range of applications in investigating real-time dynamic phenomena.

Spatiotemporal observation of light propagation in a three-dimensional scattering medium.

Abstract: Spatiotemporal information about light pulse propagation obtained with femtosecond temporal resolution plays an important role in understanding transient phenomena and light-matter interactions. Although ultrafast optical imaging techniques have been developed, it is still difficult to capture light pulse propagation spatiotemporally. Furthermore, imaging through a three-dimensional (3-D) scattering medium is a longstanding challenge due to the optical scattering caused by the interaction between light pulse and a 3-D scattering medium. Here, we propose a technique for ultrafast optical imaging of light pulses propagating inside a 3D scattering medium. We record an image of the light pulse propagation using the ultrashort light pulse even when the interaction between light pulse and a 3-D scattering medium causes the optical scattering. We demonstrated our proposed technique by recording converging, refracted, and diffracted propagating light for 59 ps with femtosecond temporal resolution.

Highly stable digital holography for temperature measurement

Abstract: We have developed a common-path configuration of digital holography offering several advantages in comparison to two-channel configurations of digital holography. This system shows a very high temporal phase stability which makes it more appropriate for real-time and fast transient dynamic measurement applications, the configuration is very simple and the system is very compact in size, and it is less vibration sensitive. Moreover, with the full use of the reference beam, the proposed system provides the field of view (FOV) as wide as that of a two-beam interferometer. The proposed system is used for the measurement of the refractive index and temperature inside the candle flame. A complete analysis of the temperature measurement from the recorded digital hologram in the presence of a candle flame is demonstrated. The advantages and limitations of the systems are also discussed.

Decoupling the refractive index and thickness by dual-wavelength digital holographic microscopy

Abstract: We have proposed a new configuration of the single-shot, dual-wavelength, common-path, off-axis DHM system. The proposed system is used for decoupling the refractive index and thickness of a biological specimen.

Binocular dynamic holographic floating image display.

Abstract: This paper proposes a binocular holographic floating display. The device consists of two phase-modulation spatial light modulators (SLM) and a dihedral corner reflector array (DCRA) element. The conjugate images of the SLMs generated by the DCRA become the system’s exit pupils. Exit pupils that are larger than the pupils of human eyes are arranged to locate at the position of observer's eyes. Therefore, the dimension of the SLM will not limit the viewing angle, although the pixel pitch of the SLM still limits the maximum field of view. For the laser light source, the resolution of the images can achieve 3 arc minutes when the distance between images and DCRA is less than 20 cm. The full-color display function is also performed in the proposed device.