Pascale Daran-Lapujade

TL;DR: The versatility of CRISPR/Cas9-based engineering of yeast is demonstrated by achieving simultaneous integration of a multigene construct combined with gene deletion and the simultaneous introduction of two single-nucleotide mutations at different loci.

TL;DR: Results indicate that in vivo fluxes in the central carbon metabolism of S. cerevisiae grown in steadystate, carbon-limited chemostat cultures are controlled to a large extent via post-transcriptional mechanisms.

TL;DR: The assembled sequence reveals that CEN.PK113-7D has a mosaic genome that combines characteristics of laboratory strains and wild-industrial strains, and a significant enrichment of non-synonymous mutations in genes encoding for components of the cAMP signalling pathway.

TL;DR: A method is developed to dissect the hierarchical regulation of fluxes into contributions by transcription, translation, protein degradation, and posttranslational modification and it is found that transcription played a minor role, whereas regulation of protein synthesis or degradation was the most important.

TL;DR: A set of experiments to address intra- and interlaboratory reproducibility is highly relevant for application of DNA-microarray analysis in functional genomics and metabolic engineering and yielded over 95% agreement between the laboratories for transcripts that changed by over 2-fold, leaving only a small fraction of genes that exhibited laboratory bias.