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Pooja Sridhar
Researcher at University of Birmingham
Publications - 30
Citations - 1013
Pooja Sridhar is an academic researcher from University of Birmingham. The author has contributed to research in topics: Bacterial outer membrane & Medicine. The author has an hindex of 11, co-authored 20 publications receiving 786 citations.
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Journal ArticleDOI
A method for detergent-free isolation of membrane proteins in their local lipid environment
Sarah C. Lee,Timothy J. Knowles,Vincent L. G. Postis,Vincent L. G. Postis,Mohammed Jamshad,Rosemary A. Parslow,Yu-pin Lin,Adrian Goldman,Adrian Goldman,Pooja Sridhar,Michael Overduin,Michael Overduin,Stephen P. Muench,Timothy R. Dafforn +13 more
TL;DR: This protocol describes the preparation of styrene maleic acid (SMA) co-polymer to extract membrane proteins from prokaryotic and eukaryotic expression systems and provides a practical tool kit for those wanting to use SMALPs to study membrane proteins.
Journal ArticleDOI
Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins.
Mohammed Jamshad,Vinciane Grimard,Ilaria Idini,Timothy J. Knowles,Miriam Dowle,Naomi Schofield,Pooja Sridhar,Yu-pin Lin,Rachael Finka,Mark Wheatley,Owen R.T. Thomas,Richard E. Palmer,Michael Overduin,Cédric Govaerts,Jean Marie Ruysschaert,Karen J. Edler,Timothy R. Dafforn +16 more
TL;DR: The structure of the SMA lipid particle (SMALP) is described using data from small angle neutron scattering, electron microscopy, and attenuated total reflection Fourier transform infrared spectroscopy to form the foundation for future development and applications of SMALPs in membrane protein production and analysis.
Journal ArticleDOI
Structure and function of BamE within the outer membrane and the β-barrel assembly machine.
Timothy J. Knowles,Douglas F. Browning,Mark Jeeves,Riyaz Maderbocus,Sandya Rajesh,Pooja Sridhar,Eleni Manoli,Danielle Emery,Ulf Sommer,Ashley H. Spencer,Denisse L. Leyton,Derrick J. P. Squire,Roy R. Chaudhuri,Mark R. Viant,Adam F. Cunningham,Ian R. Henderson,Michael Overduin +16 more
TL;DR: The native structure and mechanism of a core component of this Bam complex, BamE, are reported and it is shown that it is exclusively monomeric in its native environment of the periplasm, but is able to adopt a distinct dimeric conformation in the cytoplasm.
Journal ArticleDOI
An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles
Stephen Hall,Cecilia Tognoloni,Jack Charlton,Eilis C. Bragginton,Alice Rothnie,Pooja Sridhar,Mark Wheatley,Timothy J. Knowles,Thomas Arnold,Thomas Arnold,Karen J. Edler,Timothy R. Dafforn +11 more
TL;DR: Positively charged poly(styrene-co-maleimide) extracts functional membrane proteins into nanodiscs, overcoming some limitations of current nanodisco technology.
Journal ArticleDOI
Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system
Gareth W. Hughes,Stephen Hall,Claire S. Laxton,Pooja Sridhar,Amirul H. Mahadi,Caitlin Hatton,Thomas J. Piggot,Thomas J. Piggot,Peter J. Wotherspoon,Aneika C. Leney,Douglas G. Ward,Mohammed Jamshad,Vaclav Spana,Ian T. Cadby,Christopher J. Harding,Georgia L. Isom,Jack A. Bryant,Rebecca J. Parr,Yasin Yakub,Mark Jeeves,Damon Huber,Ian R. Henderson,Luke A. Clifton,Andrew L. Lovering,Timothy J. Knowles +24 more
TL;DR: The crystal structure of MlaC is solved in its phospholipid-free closed apo conformation, revealing a pivoting β-sheet mechanism that functions to open and close the phospholipsid-binding pocket and suggesting that the Mla pathway may have a role in anterograde phospholIPid transport.