V
Vincent L. G. Postis
Researcher at University of Leeds
Publications - 36
Citations - 1380
Vincent L. G. Postis is an academic researcher from University of Leeds. The author has contributed to research in topics: Membrane protein & Membrane. The author has an hindex of 14, co-authored 36 publications receiving 1127 citations. Previous affiliations of Vincent L. G. Postis include Joseph Fourier University & University of Bordeaux.
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Journal ArticleDOI
A method for detergent-free isolation of membrane proteins in their local lipid environment
Sarah C. Lee,Timothy J. Knowles,Vincent L. G. Postis,Vincent L. G. Postis,Mohammed Jamshad,Rosemary A. Parslow,Yu-pin Lin,Adrian Goldman,Adrian Goldman,Pooja Sridhar,Michael Overduin,Michael Overduin,Stephen P. Muench,Timothy R. Dafforn +13 more
TL;DR: This protocol describes the preparation of styrene maleic acid (SMA) co-polymer to extract membrane proteins from prokaryotic and eukaryotic expression systems and provides a practical tool kit for those wanting to use SMALPs to study membrane proteins.
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Crystal structure of a prokaryotic homologue of the mammalian oligopeptide–proton symporters, PepT1 and PepT2
Simon Newstead,David A. Drew,Alexander D. Cameron,Vincent L. G. Postis,Xiaobing Xia,Philip W. Fowler,Jean C. Ingram,Elisabeth P. Carpenter,Mark S.P. Sansom,Michael J. McPherson,Stephen A. Baldwin,So Iwata +11 more
TL;DR: The crystal structure of PepTSo is presented, a functionally similar prokaryotic homologue of the mammalian peptide transporters from Shewanella oneidensis, which reveals a ligand‐bound occluded state for the MFS and provides new insights into a general transport mechanism.
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The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy
Vincent L. G. Postis,Shaun Rawson,Jennifer Katie Mitchell,Sarah C. Lee,Rosemary A. Parslow,Timothy R. Dafforn,Stephen A. Baldwin,Stephen P. Muench +7 more
TL;DR: It is shown how a SMALP scaffold can be used to visualise membrane proteins, embedded in a near-native lipid environment, by negative stain electron microscopy, yielding structures at a modest resolution in a short (days) timeframe.
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Intrinsic acyl-CoA thioesterase activity of a peroxisomal ATP binding cassette transporter is required for transport and metabolism of fatty acids.
Carine De Marcos Lousa,Carlo W.T. van Roermund,Vincent L. G. Postis,Daniela Dietrich,Ian D. Kerr,Ronald J.A. Wanders,Stephen A. Baldwin,Alison Baker,Frederica L. Theodoulou +8 more
TL;DR: CTS, and most likely the other ABCD family members, represent rare examples of polytopic membrane proteins with an intrinsic additional enzymatic function that may regulate the entry of substrates into the β-oxidation pathway.
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Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure
Mayuriben J. Parmar,Shaun Rawson,Charlotte A. Scarff,Adrian Goldman,Timothy R. Dafforn,Stephen P. Muench,Vincent L. G. Postis,Vincent L. G. Postis +7 more
TL;DR: The structure of the Escherichia coli multidrug efflux transporter AcrB in a SMALP scaffold to sub-nm resolution is reported, with the resulting map being consistent with high resolution crystal structures and other EM derived maps.