R
Ritsuko Arai
Researcher at Fukushima Medical University
Publications - 13
Citations - 808
Ritsuko Arai is an academic researcher from Fukushima Medical University. The author has contributed to research in topics: Schizosaccharomyces pombe & Hydrostatic pressure. The author has an hindex of 5, co-authored 13 publications receiving 727 citations. Previous affiliations of Ritsuko Arai include National Institute of Genetics.
Papers
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Journal ArticleDOI
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe
Akihisa Matsuyama,Ritsuko Arai,Yoko Yashiroda,Atsuko Shirai,Ayako Kamata,Shigeko Sekido,Yumiko Kobayashi,Atsushi Hashimoto,Makiko Hamamoto,Yasushi Hiraoka,Sueharu Horinouchi,Minoru Yoshida +11 more
TL;DR: A proteome-scale study of the fission yeast Schizosaccharomyces pombe based on cloning of the ORFeome is reported, obtaining 4,910 ORFs in a form that is readily usable in various analyses.
Journal ArticleDOI
Global Analysis of Gel Mobility of Proteins and Its Use in Target Identification
Atsuko Shirai,Akihisa Matsuyama,Yoko Yashiroda,Atsushi Hashimoto,Yumi Kawamura,Ritsuko Arai,Yasuhiko Komatsu,Sueharu Horinouchi,Minoru Yoshida +8 more
TL;DR: The general usefulness of the mobilitome and electrophoresis-based methodology for the identification and characterization of proteins detected on the gel is proposed and previously unknown post-translational modifications of a protein are detected.
Journal ArticleDOI
A Genetically Encoded Probe for Live-Cell Imaging of H4K20 Monomethylation
Yuko Sato,Tomoya Kujirai,Ritsuko Arai,Haruhiko Asakawa,Chizuru Ohtsuki,Naoki Horikoshi,Kazuo Yamagata,Jun Ueda,Takahiro Nagase,Tokuko Haraguchi,Yasushi Hiraoka,Akatsuki Kimura,Hitoshi Kurumizaka,Hiroshi Kimura +13 more
TL;DR: A new mintbody specific for histone H4 Lys20 monomethylation (H4K20me1) is developed, which provides a powerful tool to track this particular histone modification in living cells and organisms.
Journal ArticleDOI
Reduction in chromosome mobility accompanies nuclear organization during early embryogenesis in Caenorhabditis elegans.
Ritsuko Arai,Ritsuko Arai,Takeshi Sugawara,Takeshi Sugawara,Yuko Sato,Yohei Minakuchi,Atsushi Toyoda,Kentaro Nabeshima,Hiroshi Kimura,Akatsuki Kimura,Akatsuki Kimura +10 more
TL;DR: It is proposed that the earliest global transformation in nuclear organization occurs at the 8-cell stage during C. elegans embryogenesis, which is similar to how chromosomes are packed in undifferentiated cells and how nuclear organization changes during development.
Book ChapterDOI
Improved Electron Microscopy Fixation Methods for Tracking Autophagy-Associated Membranes in Cultured Mammalian Cells.
Ritsuko Arai,Satoshi Waguri +1 more
TL;DR: In this article, the authors describe fixation procedures using (1) ferrocyanide-reduced osmium for CLEM and (2) aldehyde/OsO4 mixture for detecting omegasome structures and isolation membrane-associated tubules.