R
Rob W.H. Ruigrok
Researcher at Centre national de la recherche scientifique
Publications - 171
Citations - 14718
Rob W.H. Ruigrok is an academic researcher from Centre national de la recherche scientifique. The author has contributed to research in topics: Nucleoprotein & RNA. The author has an hindex of 70, co-authored 166 publications receiving 13831 citations. Previous affiliations of Rob W.H. Ruigrok include National Institute for Medical Research & European Bioinformatics Institute.
Papers
More filters
Journal ArticleDOI
The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit
Alexandre Dias,Denis Bouvier,Thibaut Crépin,Andrew A. McCarthy,Darren J. Hart,Florence Baudin,Stephen Cusack,Rob W.H. Ruigrok +7 more
TL;DR: In this paper, the amino-terminal 209 residues of the PA subunit contain the active site of the endonuclease active site, which is shown to be strongly activated by manganese ions, matching observations reported for the intact trimeric polymerase.
Journal ArticleDOI
The Structural Basis for CAP Binding by Influenza Virus Polymerase Subunit Pb2.
Delphine Guilligay,Franck Tarendeau,Patricia Resa-Infante,Rocío Coloma,Thibaut Crépin,Peter Sehr,Joe Lewis,Rob W.H. Ruigrok,Juan Ortín,Darren J. Hart,Stephen Cusack +10 more
TL;DR: Binding and functional studies with point mutants confirm that the identified site is essential for cap binding in vitro and cap-dependent transcription in vivo by the trimeric polymerase complex, and will allow efficient structure-based design of new anti-influenza compounds inhibiting viral transcription.
Journal ArticleDOI
A structural model for unfolded proteins from residual dipolar couplings and small-angle x-ray scattering
Pau Bernadó,Laurence Blanchard,Peter A. Timmins,Dominique Marion,Rob W.H. Ruigrok,Martin Blackledge +5 more
TL;DR: The demonstration that conformational behavior of unfolded proteins can be accurately predicted from the primary sequence by using a simple set of rules has important consequences for the understanding of the structure and dynamics of the unstructured state.
Journal ArticleDOI
Structural insight into cap-snatching and RNA synthesis by influenza polymerase
Stefan Reich,Delphine Guilligay,Alexander Pflug,Hélène Malet,Imre Berger,Thibaut Crépin,Darren J. Hart,Thomas Lunardi,Max H. Nanao,Rob W.H. Ruigrok,Stephen Cusack +10 more
TL;DR: Crystal structures of bat influenza A and human influenza B polymerases (FluA and FluB), bound to the viral RNA promoter, are used to give mechanistic insight into these distinct processes.
Journal ArticleDOI
Crystal Structure of the Rabies Virus Nucleoprotein-RNA Complex
Aurélie A. V. Albertini,Amy K. Wernimont,Tadeusz Muzioł,Raimond B. G. Ravelli,Cedric R. Clapier,Guy Schoehn,Winfried Weissenhorn,Rob W.H. Ruigrok +7 more
TL;DR: RNA sequestering by nucleoproteins is likely a common mechanism used by negative-strand RNA viruses to protect their genomes from the innate immune response directed against viral RNA in human host cells at certain stages of an infectious cycle.