R
Robert D. Stedtfeld
Researcher at Michigan State University
Publications - 74
Citations - 5638
Robert D. Stedtfeld is an academic researcher from Michigan State University. The author has contributed to research in topics: Loop-mediated isothermal amplification & Resistome. The author has an hindex of 27, co-authored 73 publications receiving 4231 citations. Previous affiliations of Robert D. Stedtfeld include University of Southern Mississippi.
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Journal ArticleDOI
Diverse and abundant antibiotic resistance genes in Chinese swine farms.
Yong-Guan Zhu,Timothy A. Johnson,Jian-Qiang Su,Min Qiao,Guang Xia Guo,Robert D. Stedtfeld,Syed A. Hashsham,James M. Tiedje +7 more
TL;DR: Diverse, abundant, and potentially mobile ARGs in farm samples suggest that unmonitored use of antibiotics and metals is causing the emergence and release of ARGs to the environment.
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In-feed antibiotic effects on the swine intestinal microbiome.
Torey Looft,Timothy A. Johnson,Heather K. Allen,Darrell O. Bayles,David P. Alt,Robert D. Stedtfeld,Woo Jun Sul,Tiffany M. Stedtfeld,Benli Chai,James R. Cole,Syed A. Hashsham,James M. Tiedje,Thad B. Stanton +12 more
TL;DR: Analysis of the metagenomes showed that microbial functional genes relating to energy production and conversion were increased in the antibiotic-fed pigs and that antibiotic resistance genes increased in abundance and diversity in the medicated swine microbiome despite a high background of resistance genes in nonmedication swine.
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Antibiotic resistance in European wastewater treatment plants mirrors the pattern of clinical antibiotic resistance prevalence
Katariina Pärnänen,Carlos Narciso-da-Rocha,David Kneis,Thomas U. Berendonk,Damiano Cacace,Thi Thuy Do,Christian Elpers,Despo Fatta-Kassinos,Isabel Henriques,Thomas Jaeger,Antti Karkman,José L. Martínez,Stella Michael,I. Michael-Kordatou,Kristin O'Sullivan,Sara Rodríguez-Mozaz,Thomas Schwartz,Hongjie Sheng,Hongjie Sheng,Henning Sørum,Robert D. Stedtfeld,James M. Tiedje,Saulo Varela Della Giustina,Fiona Walsh,Ivone Vaz-Moreira,Marko Virta,Célia M. Manaia +26 more
TL;DR: This first trans-Europe surveillance showed that UWTP AR profiles mirror the AR gradient observed in clinics, and the need to implement regular surveillance and control measures, which may need to be appropriate for the geographic regions, is highlighted.
Journal ArticleDOI
Gene-Z: a device for point of care genetic testing using a smartphone.
Robert D. Stedtfeld,Dieter M. Tourlousse,Gregoire Seyrig,Tiffany M. Stedtfeld,Maggie Kronlein,Scott Price,Farhan Ahmad,Erdogan Gulari,James M. Tiedje,Syed A. Hashsham +9 more
TL;DR: The Gene-Z represents a significant step toward truly inexpensive and compact tools for POC genetic testing, including sensitivity and reproducibility using genomic DNA from Escherichia coli and Staphylococcus aureus.
Journal ArticleDOI
High-throughput quantification of antibiotic resistance genes from an urban wastewater treatment plant.
Antti Karkman,Timothy A. Johnson,Christina Lyra,Robert D. Stedtfeld,Manu Tamminen,James M. Tiedje,Marko Virta +6 more
TL;DR: Wastewater discharge enriched only a few genes, namely Tn25 type transposase gene and clinical class 1 integrons, in the sediment near the discharge pipe, but those enriched genes may indicate a potential for horizontal gene transfer.