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Ryuji J. Machida
Researcher at Academia Sinica
Publications - 46
Citations - 2276
Ryuji J. Machida is an academic researcher from Academia Sinica. The author has contributed to research in topics: Biology & Gene. The author has an hindex of 21, co-authored 40 publications receiving 1776 citations. Previous affiliations of Ryuji J. Machida include National Taiwan Normal University & National Museum of Natural History.
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A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents
Matthieu Leray,Matthieu Leray,Joy Y. Yang,Christopher P. Meyer,Suzanne C. Mills,Natalia Agudelo,Vincent Ranwez,J. T. Boehm,J. T. Boehm,Ryuji J. Machida +9 more
TL;DR: The molecular analysis of gut contents targeting the 313 COI fragment using the newly designed mlCOIintF primer in combination with the jgHCO2198 primer offers enormous promise for metazoan metabarcoding studies.
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Metazoan mitochondrial gene sequence reference datasets for taxonomic assignment of environmental samples.
TL;DR: All metazoan mitochondrial gene sequences from GenBank are retrieved, and quality filtered and formatted the datasets for taxonomic assignments using taxonomic assignment tools, and the mitochondrial Cytochrome oxidase subunit I gene was the most sequence-rich gene.
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Complete mitochondrial DNA sequence of Tigriopus japonicus (Crustacea: Copepoda).
TL;DR: The complete nucleotide sequence of the mitochondrial genome was determined for a harpacticoid copepod, Tigriopus japonicus (Crustacea), using an approach that employs a long polymerase chain reaction technique and primer walking, resulting in one of the smallest mitochondrial genomes in the arthropod lineage.
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GenBank is a reliable resource for 21st century biodiversity research
TL;DR: Encouraging results suggest that the rapid uptake of DNA-based approaches is supported by a bioinformatic infrastructure capable of assessing both the losses to biodiversity caused by global change and the effectiveness of conservation efforts aimed at slowing or reversing these losses.
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Evidence for the grazing hypothesis: Grazing reduces phytoplankton responses of the HNLC ecosystem to iron enrichment in the western subarctic pacific (SEEDS II)
Atsushi Tsuda,Shigenobu Takeda,Hiroaki Saito,Jun Nishioka,Isao Kudo,Yukihiro Nojiri,Koji Suzuki,Mitsuo Uematsu,Mark L. Wells,Daisuke Tsumune,Takeshi Yoshimura,Tatsuo Aono,Takafumi Aramaki,William P. Cochlan,Maki Hayakawa,Keiri Imai,Tomoshi Isada,Yoko Iwamoto,W.K. Johnson,Sohiko Kameyama,Shungo Kato,Hiroshi Kiyosawa,Yoshiko Kondo,Maurice Levasseur,Ryuji J. Machida,Ippei Nagao,Fumiko Nakagawa,Takahiro Nakanishi,Seiji Nakatsuka,Akira Narita,Yoshifumi Noiri,Hajime Obata,Hiroshi Ogawa,Kenji Oguma,Tsuneo Ono,tomofumi sakuragi,Motoki Sasakawa,Mitsuhide Sato,Akifumi Shimamoto,Hyoe Takata,Charles G. Trick,Yutaka W. Watanabe,Chi Shing Wong,Naoki Yoshie +43 more
TL;DR: The grazing rate estimation indicates that the copepod grazing prevented the formation of an extensive diatom bloom, which was observed in SEEDS, and led to the change to a pico-phytoplankton dominated community towards the end of the experiment.