Sarantos Kyriakopoulos

TL;DR: The human genome-scale metabolic reconstruction details all known metabolic reactions occurring in humans, and thereby holds substantial promise for studying complex diseases and phenotypes as discussed by the authors, and since the last community effort generated a consensus reconstruction, several updates have been developed.

TL;DR: Through updates, the most complete and best annotated consensus human metabolic reconstruction available is achieved, thereby increasing the ability of this resource to provide novel insights into normal and disease states in human.

TL;DR: The models accurately predict growth phenotypes and known auxotrophies in CHO cells and show that the metabolic resources in CHO are more than three times more efficiently utilized for growth or recombinant protein synthesis following targeted efforts to engineer the CHO secretory pathway.

TL;DR: The recent availability of abundant high‐throughput multi‐omics datasets from mammalian cell cultures have now paved the way to improve parameterization of kinetic models, and integrate regulatory, signaling, and product quality related intracellular events, as well as cellular metabolism within the modeling framework.

TL;DR: A global transcriptome and proteome profiling of three commonly used parental cell lines in suspension cultures is presented and their growth‐related characteristics, and N‐ and O‐glycosylation patterns of host cell proteins (HCPs) are reported.