S
Shengchao Shi
Researcher at Nanjing University
Publications - 13
Citations - 439
Shengchao Shi is an academic researcher from Nanjing University. The author has contributed to research in topics: Chemistry & Force spectroscopy. The author has an hindex of 6, co-authored 10 publications receiving 125 citations.
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Journal ArticleDOI
N501Y mutation of spike protein in SARS-CoV-2 strengthens its binding to receptor ACE2.
Fang Tian,Bei Tong,Liang Sun,Shengchao Shi,Bin Zheng,Zibin Wang,Xianchi Dong,Xianchi Dong,Peng Zheng +8 more
TL;DR: In this paper, the authors used a cell surface-binding assay, a kinetics study, a single-molecule technique, and a computational method to investigate the interaction between these RBD (mutations) and ACE2.
Posted ContentDOI
Mutation N501Y in RBD of Spike Protein Strengthens the Interaction between COVID-19 and its Receptor ACE2
TL;DR: Wang et al. as discussed by the authors combined cell surface binding assay, kinetics study, single-molecule technique, and computational method to investigate the interaction between these RBD (mutations) and ACE2.
Journal ArticleDOI
Enzymatic biosynthesis and immobilization of polyprotein verified at the single-molecule level.
Yibing Deng,Tao Wu,Mengdi Wang,Shengchao Shi,Guodong Yuan,Xi Li,Hanchung Chong,Bin Wu,Peng Zheng +8 more
TL;DR: An enzymatic procedure to build polyprotein using the combination of a strict protein ligase OaAEP1 (Oldenlandia affinis asparaginyl endopeptidases 1) and a protease TEV (tobacco etch virus) and the feasibility of synthesizing protein polymers with rationally-controlled sequences by the synergy of the ligase and protease is shown.
Journal ArticleDOI
Combination of Click Chemistry and Enzymatic Ligation for Stable and Efficient Protein Immobilization for Single-Molecule Force Spectroscopy
TL;DR: Protein immobilization is an essential method for both basic and applied research into protein, and the covalent, site-specific attachment is the most desirable strategy.
Journal ArticleDOI
Verification of sortase for protein conjugation by single-molecule force spectroscopy and molecular dynamics simulations.
TL;DR: The use of sortase for protein conjugation is verified, and a longer linker with a higher glycine content should be used with caution, because of the high probability of a closed conformation.