Shuye Pu

TL;DR: T tandem affinity purification was used to process 4,562 different tagged proteins of the yeast Saccharomyces cerevisiae to identify protein–protein interactions, which will help future studies on individual proteins as well as functional genomics and systems biology.

TL;DR: Two catalogues of yeast protein complexes are reported, one of which is a comprehensive catalogue of 408 manually curated heteromeric protein complexes reliably backed by small-scale experiments reported in the current literature and the other comprises 400 high-throughput complexes annotated with current literature evidence.

TL;DR: A complete survey of putative integral, peripheral and lipid-anchored MPs from Saccharomyces cerevisiae was reported, which revealed unexpected physical associations underlying the membrane biology of eukaryotes and delineates the global topological landscape of the membrane interactome.

TL;DR: This work applies the Markov Cluster procedure to an alternative yeast interaction network, recently derived by combining the data from the two latest TAP/MS studies, and produces a new description of yeast protein complexes that is more accurate and meaningful than those previously published.

TL;DR: This paper quantified the abundance of all the proteins in immunoprecipitates of 1,124 new recombinant antibodies for 152 chromatin-related human proteins by comparing normalized spectral abundance factors from the target antigen with those of all other proteins.