Siegfried Knasmueller

TL;DR: This protocol describes one of the current established methods for buccal cell collection using a small-headed toothbrush, the generation of a single-cell suspension, slide preparation using cytocentrifugation, fixation and staining using Feulgen and Light Green for both bright field and fluorescence microscopic analysis.

TL;DR: The harmonization and standardization of the buccal MN assay will allow more reliable comparison of the data among human populations and laboratories, evaluation of the assay's performance, and consolidation of its world-wide use for biomonitoring of DNA damage.

TL;DR: The results of the HUMN(XL) project identified priorities for validation studies, increased the basic knowledge of the assay, and contributed to the creation of a laboratory network which in perspective may allow the evaluation of disease risk associated with MN frequency.

TL;DR: The results obtained show that both under cell‐free conditions and in the presence of cells the oxidant activities of GTE and EGCG exceeded those of spontaneously generated H2O2 (FOX assay), and suggest that detailed mechanistic studies on the effects of GTe and E GCG should be performed in vivo before excessive intake and/ or topical application of green tea products can be recommended to healthy and/or diseased persons.

TL;DR: The central aims were to collect databases on baseline MN frequencies and associated methodological, demographic, genetic and exposure variables, determine those variables that affect MN frequency, establish standardised protocols for performing assays, and evaluate the association of MN frequency with disease outcomes both cross-sectionally and prospectively.