Claudia Bolognesi

TL;DR: Preliminary evidence is provided that MN frequency in PBL is a predictive biomarker of cancer risk within a population of healthy subjects and in all national cohorts and for all major cancer sites.

TL;DR: The majority of studies on cytogenetic biomarkers in pesticide-exposed workers have indicated some dose-dependent effects, with increasing duration or intensity of exposure, but data at present on the effect of genetic polymorphism on susceptibility to pesticides does not allow any conclusion.

TL;DR: A selection of biomarkers of stress, genotoxicity and exposure usually employed in biomonitoring programmes is presented, together with a brief overview of new biomolecular approaches.

TL;DR: This protocol describes one of the current established methods for buccal cell collection using a small-headed toothbrush, the generation of a single-cell suspension, slide preparation using cytocentrifugation, fixation and staining using Feulgen and Light Green for both bright field and fluorescence microscopic analysis.

TL;DR: The harmonization and standardization of the buccal MN assay will allow more reliable comparison of the data among human populations and laboratories, evaluation of the assay's performance, and consolidation of its world-wide use for biomonitoring of DNA damage.