S
Spencer T. Adams
Researcher at University of Massachusetts Medical School
Publications - 10
Citations - 481
Spencer T. Adams is an academic researcher from University of Massachusetts Medical School. The author has contributed to research in topics: Luciferin & Luciferase. The author has an hindex of 8, co-authored 9 publications receiving 392 citations.
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Journal ArticleDOI
A synthetic luciferin improves bioluminescence imaging in live mice.
Melanie S. Evans,Joanna P Chaurette,Spencer T. Adams,Gadarla Randheer Reddy,Miranda A. Paley,Neil Aronin,Jennifer A. Prescher,Stephen C. Miller +7 more
TL;DR: Injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.
Journal ArticleDOI
Beyond D-luciferin: Expanding the Scope of Bioluminescence Imaging in vivo
TL;DR: Recent work to replace the natural luciferase substrate with synthetic analogs that extend the scope of bioluminescence imaging is described.
Journal ArticleDOI
Luciferin Amides Enable in Vivo Bioluminescence Detection of Endogenous Fatty Acid Amide Hydrolase Activity.
David M. Mofford,Spencer T. Adams,G. S. Kiran Kumar Reddy,Gadarla Randheer Reddy,Stephen C. Miller +4 more
TL;DR: Firefly luciferase substrate d-luciferin and its analogs are fatty acid mimics that are ideally suited to probe the chemistry of enzymes that release fatty acid products and serve as exemplary reagents for greatly improved bioluminescence imaging in FAAH-expressing tissues such as the brain.
Journal ArticleDOI
Firefly Luciferase Mutants Allow Substrate‐Selective Bioluminescence Imaging in the Mouse Brain
TL;DR: It is shown that mutants of firefly luciferase can discriminate between natural and synthetic substrates in the brains of live mice and that mutant luciferases that are inactive or weakly active with d-luciferin can light up brightly when treated with the aminoluciferins CycLuc1 and Cyc Luc2 or their respective FAAH-sensitive luciferin amides.
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Rapid Access to a Broad Range of 6'-Substituted Firefly Luciferin Analogues Reveals Surprising Emitters and Inhibitors.
TL;DR: Two-step route to a broad range of 6'-substituted luciferin analogues was developed to enable more extensive study of the 6'-functionality and revealed thioether inhibitors and unexpectedly luminogenic aryl amine derivatives.