A synthetic luciferin improves bioluminescence imaging in live mice.
Melanie S. Evans,Joanna P Chaurette,Spencer T. Adams,Gadarla Randheer Reddy,Miranda A. Paley,Neil Aronin,Jennifer A. Prescher,Stephen C. Miller +7 more
TLDR
Injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.Abstract:
Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.read more
Citations
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Journal ArticleDOI
Single-cell bioluminescence imaging of deep tissue in freely moving animals
Satoshi Iwano,Mayu Sugiyama,Hiroshi Hama,Akiya Watakabe,Naomi Hasegawa,Takahiro Kuchimaru,Kazumasa Z. Tanaka,Megumu Takahashi,Yoko Ishida,Jun-ichi Hata,Satoshi Shimozono,Kana Namiki,Takashi Fukano,Masahiro Kiyama,Hideyuki Okano,Shinae Kizaka-Kondoh,Thomas J. McHugh,Tetsuo Yamamori,Hiroyuki Hioki,Shojiro Maki,Atsushi Miyawaki +20 more
TL;DR: AkaBLI produced emissions in vivo that were brighter by a factor of 100 to 1000 than conventional systems, allowing noninvasive visualization of single cells deep inside freely moving animals, and is therefore a bioengineered light source to spur unprecedented scientific, medical, and industrial applications.
Journal ArticleDOI
1001 lights: luciferins, luciferases, their mechanisms of action and applications in chemical analysis, biology and medicine
Zinaida M. Kaskova,Zinaida M. Kaskova,Aleksandra S. Tsarkova,Aleksandra S. Tsarkova,Ilia V. Yampolsky,Ilia V. Yampolsky +5 more
TL;DR: The properties and applications of d-luciferin, coelenterazine, bacterial, Cypridina and dinoflagellate luciferins and their analogues along with their corresponding luciferases are described.
Journal ArticleDOI
In Vivo Molecular Bioluminescence Imaging: New Tools and Applications
TL;DR: In vivo bioluminescence imaging is an optical molecular imaging technique used to visualize molecular and cellular processes in health and diseases and to follow the fate of cells with high sensitivity using luciferase-based gene reporters.
Journal ArticleDOI
A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging
Takahiro Kuchimaru,Satoshi Iwano,Masahiro Kiyama,Shun Mitsumata,Tetsuya Kadonosono,Haruki Niwa,Shojiro Maki,Shinae Kizaka-Kondoh +7 more
TL;DR: The bioluminescence produced by AkaLumine-HCl in reactions with native firefly luciferase is in the near-infrared wavelength ranges, and yields significantly increased target-detection sensitivity from deep tissues with maximal signals attained at very low concentrations, as compared with D-luciferin and emerging synthetic luciferin CycLuc1.
Journal ArticleDOI
Red-shifted luciferase–luciferin pairs for enhanced bioluminescence imaging
Hsien-Wei Yeh,Omran Karmach,Ao Ji,David Carter,Manuela Martins-Green,Hui-wang Ai,Hui-wang Ai +6 more
TL;DR: The development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence are described and one pair showed superior in vitro and in vivo sensitivity over commonly used biolumeinescence reporters is adapted.
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