Stephen J. Hattan

TL;DR: It is found that inactivation of Upf1p and Xrn1p causes common as well as unique effects on protein expression, and the use of 4-fold multiplexing to enable relative protein measurements simultaneously with determination of absolute levels of a target protein using synthetic isobaric peptide standards.

TL;DR: The preliminary findings of iTRAQ suggest that a roster of proteins may be generated and developed into specific biomarkers that could eventually assist in clinical diagnosis and monitoring disease progression of AD, PD and DLB.

TL;DR: Circular dichroism shows that the protein-calcium binding/protomer formation is coupled to a significant rearrangement in the protein's secondary structure in which there is a major reduction in β-sheet with an associated increase in α-helical content of the protein.

TL;DR: There is substantial potential for MALDI-TOF MS to make further inroads into clinical chemistry because of the selectivity of mass detection and its ability to independently quantify proteoforms.

TL;DR: The feasibility of MALDI MS/MS with off-line coupling to HPLC for the analysis of whole cell lysates of wild-type yeast by three different workflows is demonstrated and the effect of a workflow on the end results in terms of the number of proteins detected, the average peptide coverage of proteins, and thenumber of redundant peptide sequencing attempts is demonstrated.