Teng Zhao

TL;DR: A novel mitochondrion-specific photo-activatable fluorescence turn-on bioprobe, named as o-TPE-ON+, is designed and readily prepared, operating through a new photoactivatable mechanism of photocyclodehydrogenation.

TL;DR: This work demonstrates a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter and obtained two-color, 3D images of biological samples with lateral/axial resolution and high-speed, 4D volume imaging of live sample at 1 Hz temporal resolution.

TL;DR: An alternative TGN-independent Golgi-to-PM polar exocytic route is demonstrated, which mediates secretion of NtPPME1 for cell wall formation during cell expansion and cytokinesis and is ROP1-dependent.

TL;DR: The data suggest that ATM and ATR are part of the cellular “infrastructure” that maintains the excitatory/inhibitory balance of the nervous system.

TL;DR: A robust two-dye combination of Alexa647 and Alexa750 in an imaging buffer containing COT and using TCEP as switching regent provides matched and balanced switching characteristics for both dyes, allowing simultaneous capture of both on a single camera.