Showing papers by "Tuhin Subhra Santra published in 2022"
TL;DR: This review highlights the fabrication of different surface-modified nanocellulose to deliver active molecules, such as drugs, proteins, and plasmids, and its composites in tissue engineering.
59 citations
TL;DR: In this article , a review highlights these unconventional responses of charge carriers and discusses the underlying physics for contemporary layered and one-dimensional materials, revealing extraordinary photophysical properties of materials which are essential for designing high-frequency advanced optoelectronic devices.
9 citations
TL;DR: In this paper , the authors highlight recent advances of microfluidic devices for single-neuron analysis, i.e., single neuron trapping, single neuron dynamics, singleneuron proteomics, drug delivery at the single neuron level, single axon guidance, and single neuron differentiation.
Abstract: Single-neuron actions are the basis of brain function, as clinical sequelae, neuronal dysfunction or failure for most of the central nervous system (CNS) diseases and injuries can be identified via tracing single-neurons. The bulk analysis methods tend to miscue critical information by assessing the population-averaged outcomes. However, its primary requisite in neuroscience to analyze single-neurons and to understand dynamic interplay of neurons and their environment. Microfluidic systems enable precise control over nano-to femto-liter volumes via adjusting device geometry, surface characteristics, and flow-dynamics, thus facilitating a well-defined micro-environment with spatio-temporal control for single-neuron analysis. The microfluidic platform not only offers a comprehensive landscape to study brain cell diversity at the level of transcriptome, genome, and/or epigenome of individual cells but also has a substantial role in deciphering complex dynamics of brain development and brain-related disorders. In this review, we highlight recent advances of microfluidic devices for single-neuron analysis, i.e., single-neuron trapping, single-neuron dynamics, single-neuron proteomics, single-neuron transcriptomics, drug delivery at the single-neuron level, single axon guidance, and single-neuron differentiation. Moreover, we also emphasize limitations and future challenges of single-neuron analysis by focusing on key performances of throughput and multiparametric activity analysis on microfluidic platforms.
5 citations
TL;DR: In this paper , the size of trapped bubbles increased with softer hydrogels, higher laser energy level, larger agglomerate size, and smaller interparticle distance among agglomers.
2 citations
TL;DR: In this paper , a thin layer of Poly Lactic-co-Glycolic Acid (PLGA) was coated on top of the drugloaded titanium-dioxide nanotubes at various thicknesses.
Abstract: Titanium-dioxide nanotubes (TNTs) were fabricated by anodic oxidation of titanium (Ti) substrate and loaded with ciprofloxacin. A thin layer of Poly Lactic-co-Glycolic Acid (PLGA) was coated on top of the drug-loaded TNTs at various thicknesses. Field Emission Scanning Electron Microscopy (FESEM) was used to characterize the developed substrate. The FESEM images of anodized samples showed that, the native oxide layer on the surface of the Ti substrate was replaced with an array of ordered nanotubes up to ~700 nm in length. Drug release studies of ciprofloxacin were conducted in Phosphate Buffered Saline (PBS) solution for 24 h. The studies showed the ability of PLGA to slow down the drug release rate, depending on the thickness of the deposited layer. MG-63 cells (human osteosarcoma cell lines) were cultured on the TNT substrates after loading ciprofloxacin and coating with various concentrations of PLGA to show the potentiality for better osseointegration. An MTT assay was carried out to study cell viability and proliferation on these substrates. Anti-microbial studies were carried out to demonstrate the release of ciprofloxacin in treating infections against Staphylococcus epidermidis. These in-depth studies showed that local concentration of the drug released could be controlled by varying the thickness of the PLGA layer to allow for better implant osseointegration and treatment of bone infections.
2 citations
TL;DR: In this article , the authors discuss different biochemical as well as physical approaches for the surface modification and cellular adhesion, and also emphasize in-depth single-cell patterning by using different physiochemical and physical approaches and their biological and biomedical applications.
1 citations
TL;DR: In this article , a review of different developments in hybrid therapeutic techniques for cellular delivery and analysis is presented, including mechano-electroporation, electro-sonoporation, magneto-mechanoporation and magnetic hyperthermia studies.
TL;DR: In this article , a symmetric flow-focusing droplet device was used to tune LSPR tunable gold nanostars (Au NS) by optimizing the flow parameters in 2D simulations.
TL;DR: In this paper , an object detection-based DL algorithm was proposed to detect single cells in microwell arrays and predicts the presence of cells in resource-limited environments at the highest possible mAP (mean average precision) of 0.989 with an average inference time of O(0.06 s).
Abstract: Abstract Single-cell analysis has been widely used in various biomedical engineering applications, ranging from cancer diagnostics, and immune response monitoring to drug screening. Single-cell isolation is fundamental for observing single-cell activities and an automatic finding method of accurate and reliable cell detection with few possible human errors is also essential. This paper reports trapping single cells into photo patternable hydrogel microwell arrays and isolating them. Additionally, we present an object detection-based DL algorithm that detects single cells in microwell arrays and predicts the presence of cells in resource-limited environments at the highest possible mAP (mean average precision) of 0.989 with an average inference time of 0.06 s. This algorithm leads to the enhancement of the high-throughput single-cell analysis, establishing high detection precision and reduced experimentation time.
TL;DR: In 1665, Robert Hooke published his revolutionary book Micrographia as discussed by the authors , which was the first publication of the first edition of the Micrographian journal Micrographias.
Abstract: In 1665, Robert Hooke published his revolutionary book Micrographia [...].
TL;DR: This Research Topic of Frontiers of Bioengineering and Biotechnology entitled “Micro/Nano Optical Devices for Biosensing and Cellular Analysis” covers the recent advancements in biosensing and cellular analysis using different approaches.
Abstract: Recent advancements in micro technologies and nanotechnologies integrated with chemical, electrical, optical, biological, and biomedical engineering, generating a new era to developed micro/nanoscale optical devices for diverse types of biomolecular sensing and cellular analysis. For biosensing and cellular analysis, optical devices such as metamaterial, metallic and semiconducting nanostructures, and plasmonic devices have sparked a lot of research interest. They exhibit great sensitivity and robustness and can potentially fit on a single chip. Because of their significant capability to control light at subwavelength scales, metamaterial and plasmonic devices have drawn enormous attention for various applications beyond conventional techniques. The efficiency of these devices as optical biosensors has proven to be worthwhile in detecting and manipulating various biomolecules. For cellular analysis in a complex micro-/nanofluidic environment, micro/nanofluidic optical devices have proven to be powerful techniques and inevitable tools, with minimum sample consumption and less contamination than bulk analysis. The ability to manipulate and detect biomolecules, bio-samples and reagents at the micro/nano scale level can satisfy the needs of diverse biosensing and cellular analysis. The devices not only perform precise cellular manipulation, separation, isolation, and lysis but can also provide cellular electrical, mechanical and optical characterization. This Research Topic of Frontiers of Bioengineering and Biotechnology entitled “Micro/Nano Optical Devices for Biosensing and Cellular Analysis” covers the recent advancements in biosensing and cellular analysis using different approaches. Broadly neutralising antibodies (bnAbs) against human coronavirus (HCoV) is a vital tool for future coronavirus pandemic planning. Sypabekova et al. suggested utilizing stateof-art fiber optic biosensors to identify broadly neutralizing antibodies (bnAbs). As diagnostic and environmental sensing tools, discovered bnAbs should be able to detect OPEN ACCESS