Yukihiro Yabuta

TL;DR: In this article, the authors provided genetic evidence that Prdm14, a PR domain-containing transcriptional regulator with exclusive expression in the germ cell lineage and pluripotent cell lines, is critical in two of these events, the reacquisition of potential pluripotency and successful epigenetic reprogramming.

TL;DR: A strategy to globally amplify mRNAs from single cells for highly quantitative high-density oligonucleotide microarray analysis that combines a small number of directional PCR cycles with subsequent linear amplification successfully detects subtle but essential differences in gene expression at the single-cell level among seemingly homogeneous cell populations is reported.

TL;DR: It is suggested the possibility that active repression of an essential enzyme and subsequent unique cellular dynamics ensures successful implementation of genome-wide epigenetic reprogramming in migrating PGCs.

TL;DR: It is shown that human induced pluripotent stem cells differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell- like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6.

TL;DR: It is shown that after specification in the blastocysts, EPI from cynomolgus monkeys (cyEPI) undergoes major transcriptome changes on implantation, and, while generating gastrulating cells, cyEPI stably maintains its transcriptome over a week, retains a unique set of pluripotency genes and acquires properties for ‘neuron differentiation’.