K
Knut Woltjen
Researcher at Kyoto University
Publications - 75
Citations - 8083
Knut Woltjen is an academic researcher from Kyoto University. The author has contributed to research in topics: Induced pluripotent stem cell & Reprogramming. The author has an hindex of 29, co-authored 69 publications receiving 7048 citations. Previous affiliations of Knut Woltjen include University of Calgary & International Practical Shooting Confederation.
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piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
Knut Woltjen,Iacovos P. Michael,Iacovos P. Michael,Paria Mohseni,Paria Mohseni,Ridham Desai,Ridham Desai,Maria Mileikovsky,Riikka H. Hämäläinen,Rebecca Cowling,Wei Wang,Pentao Liu,Marina Gertsenstein,Keisuke Kaji,Hoon Ki Sung,Andras Nagy,Andras Nagy +16 more
TL;DR: It is shown that the individual PB insertions can be removed from established iPS cell lines, providing an invaluable tool for discovery, and the traceless removal of reprogramming factors joined with viral 2A sequences delivered by a single transposon from murine iPS lines is demonstrated.
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Virus-free induction of pluripotency and subsequent excision of reprogramming factors
Keisuke Kaji,Katherine Norrby,Agnieszka Paca,Maria Mileikovsky,Paria Mohseni,Paria Mohseni,Knut Woltjen +6 more
TL;DR: It is shown that non-viral transfection of a single multiprotein expression vector, which comprises the coding sequences of c-Myc, Klf4, Oct4 and Sox2 linked with 2A peptides, can reprogram both mouse and human fibroblasts and can be removed once reprogramming has been achieved.
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Functional Genomics Reveals a BMP-Driven Mesenchymal-to-Epithelial Transition in the Initiation of Somatic Cell Reprogramming
Payman Samavarchi-Tehrani,Azadeh Golipour,Azadeh Golipour,Laurent David,Hoon Ki Sung,Tobias A. Beyer,Alessandro Datti,Knut Woltjen,Andras Nagy,Andras Nagy,Jeffrey L. Wrana,Jeffrey L. Wrana +11 more
TL;DR: A multistep mechanism that incorporates a BMP-miRNA-MET axis during somatic cell reprogramming is defined that is linked to B MP-dependent induction of miR-205 and themiR-200 family of microRNAs that are key regulators of MET.
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Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells.
Kotaro Sasaki,Shihori Yokobayashi,Tomonori Nakamura,Ikuhiro Okamoto,Yukihiro Yabuta,Kazuki Kurimoto,Hiroshi Ohta,Yoshinobu Moritoki,Yoshinobu Moritoki,Chizuru Iwatani,Hideaki Tsuchiya,Shinichiro Nakamura,Kiyotoshi Sekiguchi,Tetsushi Sakuma,Takashi Yamamoto,Takahide Mori,Knut Woltjen,Masato Nakagawa,Takuya Yamamoto,Kazutoshi Takahashi,Shinya Yamanaka,Mitinori Saitou +21 more
TL;DR: It is shown that human induced pluripotent stem cells differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell- like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6.
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Premature Termination of Reprogramming In Vivo Leads to Cancer Development through Altered Epigenetic Regulation
Kotaro Ohnishi,Kotaro Ohnishi,Katsunori Semi,Takuya Yamamoto,Masahito Shimizu,Akito Tanaka,Kanae Mitsunaga,Keisuke Okita,Kenji Osafune,Yuko Arioka,Toshiyuki Maeda,Hidenobu Soejima,Hisataka Moriwaki,Shinya Yamanaka,Knut Woltjen,Yasuhiro Yamada,Yasuhiro Yamada +16 more
TL;DR: It is demonstrated that iPSCs derived from Dox-withdrawn kidney tumor cells give rise to nonneoplastic kidney cells in mice, proving that they have not undergone irreversible genetic transformation and suggest that epigenetic regulation associated with iPSC derivation may drive development of particular types of cancer.