Institution
Center for Biofilm Engineering
About: Center for Biofilm Engineering is a based out in . It is known for research contribution in the topics: Biofilm & Population. The organization has 199 authors who have published 187 publications receiving 17511 citations. The organization is also known as: CBE & Biofilm Engineering.
Papers published on a yearly basis
Papers
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TL;DR: Current concepts of biofilm tolerance are reviewed with special emphasis on the role of the biofilm matrix and the physiology ofBiofilm-embedded cells, and the heterogeneity in metabolic and reproductive activity within a biofilm correlates with a non-uniform susceptibility of enclosed bacteria.
1,730 citations
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TL;DR: A synthetic derivate of natural furanone compounds can act as a potent antagonist of bacterial quorum sensing and inhibited virulence factor expression in a mouse pulmonary infection model.
Abstract: Traditional treatment of infectious diseases is based on compounds that kill or inhibit growth of bacteria. A major concern with this approach is the frequent development of resistance to antibiotics. The discovery of communication systems (quorum sensing systems) regulating bacterial virulence has afforded a novel opportunity to control infectious bacteria without interfering with growth. Compounds that can override communication signals have been found in the marine environment. Using Pseudomonas aeruginosa PAO1 as an example of an opportunistic human pathogen, we show that a synthetic derivate of natural furanone compounds can act as a potent antagonist of bacterial quorum sensing. We employed GeneChip® microarray technology to identify furanone target genes and to map the quorum sensing regulon. The transcriptome analysis showed that the furanone drug specifically targeted quorum sensing systems and inhibited virulence factor expression. Application of the drug to P.aeruginosa biofilms increased bacterial susceptibility to tobramycin and SDS. In a mouse pulmonary infection model, the drug inhibited quorum sensing of the infecting bacteria and promoted their clearance by the mouse immune response.
1,319 citations
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TL;DR: It is clearly, timely to ask the question "What are the essential differences between a planktonic cell growing in the conventional batch culture and a cell of the same species growing in a natural multispecies biofilm?"
Abstract: At its 1993 annual meeting, the American Society for Microbiology deemed the biofilm mode of growth to be a concept worthy of an extraordinary 4-day colloquium consisting of 52 lectures from invited speakers. Perhaps, immediately following this herculean exercise, it is germane to consider the available evidence for the importance and uniqueness of this mode of growth and to assess the consequences of this concept in modern microbiology. It is, clearly, timely to ask the question "What are the essential differences between a planktonic cell growing in the conventional batch culture and a cell of the same species growing in a natural multispecies biofilm?" This question can be asked with some urgency, because whether we intended it or not, microbiologists have actually extrapolated between laboratory cultures and real ecosystems for hundreds of years and, lately, these extrapolations appear to have become increasingly strained and inaccurate.
977 citations
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TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Abstract: The roles of slow antibiotic penetration, oxygen limitation, and low metabolic activity in the tolerance of Pseudomonas aeruginosa in biofilms to killing by antibiotics were investigated in vitro. Tobramycin and ciprofloxacin penetrated biofilms but failed to effectively kill the bacteria. Bacteria in colony biofilms survived prolonged exposure to either 10 μg of tobramycin ml−1or 1.0 μg of ciprofloxacin ml−1. After 100 h of antibiotic treatment, during which the colony biofilms were transferred to fresh antibiotic-containing plates every 24 h, the log reduction in viable cell numbers was only 0.49 ± 0.18 for tobramycin and 1.42 ± 0.03 for ciprofloxacin. Antibiotic permeation through colony biofilms, indicated by a diffusion cell bioassay, demonstrated that there was no acceleration in bacterial killing once the antibiotics penetrated the biofilms. These results suggested that limited antibiotic diffusion is not the primary protective mechanism for these biofilms. Transmission electron microscopic observations of antibiotic-affected cells showed lysed, vacuolated, and elongated cells exclusively near the air interface in antibiotic-treated biofilms, suggesting a role for oxygen limitation in protecting biofilm bacteria from antibiotics. To test this hypothesis, a microelectrode analysis was performed. The results demonstrated that oxygen penetrated 50 to 90 μm into the biofilm from the air interface. This oxic zone correlated to the region of the biofilm where an inducible green fluorescent protein was expressed, indicating that this was the active zone of bacterial metabolic activity. These results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
918 citations
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TL;DR: A novel chemical, propidium monoazide (PMA), that (like propidium iodide) is highly selective in penetrating only into 'dead' bacterial cells with compromised membrane integrity but not into live cells with intact cell membranes/cell walls.
917 citations
Authors
Showing all 199 results
Name | H-index | Papers | Citations |
---|---|---|---|
Philip S. Stewart | 80 | 199 | 39773 |
Paul Stoodley | 74 | 266 | 28138 |
Dirk de Beer | 65 | 280 | 18340 |
J. W. Costerton | 65 | 125 | 36122 |
Mark E. Shirtliff | 53 | 168 | 12368 |
Haluk Beyenal | 51 | 199 | 9302 |
Zbigniew Lewandowski | 51 | 130 | 10411 |
J. William Costerton | 50 | 110 | 23969 |
Gill G. Geesey | 46 | 132 | 10648 |
Anne K. Camper | 46 | 108 | 9786 |
Brent M. Peyton | 45 | 144 | 5970 |
Matthew W. Fields | 44 | 124 | 6043 |
Darren R. Korber | 42 | 126 | 6531 |
Timothy R. McDermott | 42 | 97 | 6038 |
Michael E. Clark | 39 | 73 | 7564 |